Negative effects of environmental antibiotics on non-target organisms were observed in studies at various levels of the biological organization. Yet, studies combining the effects at multiple levels were required to interpret their ecological frequencies in a broader context. Currently, effects of sulfamethoxazole (SMX) was studied on the amphipod Hyalella azteca which is important in ecological stability. At the biochemical level, effects on the antioxidant capacities showed stimulation with an inverse U-shaped change over the concentrations. The stimulation was greater in male than in females. Effects on the oxidative stress showed a U-shaped change which included stimulation and inhibition in males, and solely stimulation in females. The stimulation was less in males than in females. Effects on acetylcholinesterase (AChE) activities in both sexes were well correlated with those on oxidative stress (p < 0.05). At the individual level, effects on the body weight showed an inverse U-shaped change over concentrations, and the stimulation was greater in males than in females. The stimulations were significantly correlated with the male oxidative stress (p < 0.01), and male (p < 0.1) and female AChE activities (p < 0.05). Effects of SMX on the pre-copulation behavior also showed an inverse U-shaped change which correlated with male and female antioxidant capacities (p < 0.05) and the male body weight (p < 0.05). At the population level, effects on the reproduction showed an inverse U-shaped change over concentrations, and they significantly correlated with the male body weight (p < 0.05) and the pre-copulation behavior (p < 0.05). Summing up, SMX provoked simultaneous disturbances on the amphipod at multiple levels with sex-dependent responses.

Hexabromocyclododecane (HBCD) is commonly used in commercial products and factories. HBCDs can be detected in the air, bioaccumulated deposits, water, soil, sediments, and in biota and foodstuffs in the food-chain because they are not chemically bound to the polymer. We determined the levels of α-, β-, and γ-HBCDs in 270 foodstuffs and the doses of HBCDs Taiwanese are normally exposed to. We also wanted to create a strategy of risk management for HBCDs based on margins of exposure (MOE). HBCDs were frequently detectible in fish, seafood, and poultry. Their highest concentrations were in fish, oil, poultry, and livestock. The highest estimated daily intake was in 0- to 3-year-olds (1.576 ng/kg/day), and lower in 3- to 6- (1.064 ng/kg/day), 6- to 12- (0.899 ng/kg/day), and lowest in 12- to 16- (0.632 ng/kg/day) year-olds. The exposure doses to HBCDs indicated no health concern for Taiwanese. Except for fish, significant concentrations of α- and γ-HBCDs were detected in many other foodstuffs, which might indicate that exposure to HBCD is a relatively recent problem. Therefore, one goal of a management policy should be to follow-up the flow direction of HBCDs in Taiwan.

This work present aims to evaluate the effect of a conventional wastewater treatment process on the number of nanoparticles, and the role of nanoparticles as a carrier of antibiotics. A set of methods based on asymmetrical flow field flow fractionation coupled with multi-angle light scattering to separate and quantify nanoparticles in real wastewater was established. The characterization of nanoparticles was conducted by transmission electron microscopy, energy dispersive spectrometer, UV–visible spectrophotometer and three-dimensional excitation-emission matrix fluorescence spectroscopy. The adsorption of different sizes of nanoparticles separated from the real wastewater for four targeted antibiotics (sulfadiazine, ofloxacin, tylosin and tetracycline) was studied. The results show that the number of nanoparticles were increased in the wastewater treatment process and the size range between 60 and 80 nm was predominant in wastewater samples. The nanoparticles were mainly composed of O, Si, Al and Ca elements and organic components were in the size range of 0–10 nm. Targeted antibiotics were dominantly adsorbed onto nanoparticles with 60–80 nm size range at each stage. The concentrations of tetracycline adsorbed on nanoparticles were surprisingly increased in the end of the treatment process, while ofloxacin and tylosin had the completely opposite phenomenon to tetracycline. The pH and ionic strength definitely affected the aggregation of nanoparticles and interaction with the antibiotics. It is of great significance to give insights into nanoparticle-antibiotic assemblages for the effective treatment and avoiding the water risks due to nanoparticles’ ubiquitous and their risks of carrying antibiotics.

Carbon nanotubes (CNTs) inevitably enter domestic sewage and industrial wastewater with the continuous increase of their production and application field. The potential effect of CNTs on biological wastewater treatment processes has raised wide concerns due to their biotoxicity. In the present study, the performance, microbial community and enzymatic activity of sequencing batch reactors (SBRs) were evaluated under 148-day exposure of amino-functionalized multi-walled CNTs (MWCNTs-NH₂) at 10 and 30 mg/L. The COD removal efficiency at 10 and 30 mg/L MWCNTs-NH₂ gradually reduced from 91.03% and 90.43% on day to 89.11% and 86.70% on day 148, respectively. The NH₄⁺-N removal efficiency at 10 and 30 mg/L MWCNTs-NH₂ gradually reduced from 98.98% and 98.46% on day 1 to 96.65% and 63.39% on day 148, respectively. Compared to 0 mg/L MWCNTs-NH₂, the oxygen-utilizing rate, ammonia-oxidizing rate, nitrite-oxidizing rate, nitrite-reducing rate and nitrate-reducing rate at 30 mg/L MWCNTs-NH₂ were decreased by 52.35%, 60.58%, 55.12%, 56.56% and 57.42% on day 148, respectively. The microbial reactive oxygen species and lactate dehydrogenase release on day 148 was increased by 59.71% and 55.28% at 30 mg/L MWCNTs-NH₂, respectively. The key microbial enzymatic activity related to nitrogen removal decreased with the increase of operation time under MWCNTs-NH₂ stress. The relative abundances of Nitrosomonas, Nitrosospira, Nitrospira and some denitrifying bacteria at 10 mg/L MWCNTs-NH₂ gradually reduced with an increment in operation time. The changes of nitrogen removal rate, microbial community and enzymatic activity of SBR were related to the time-cumulative nonlinear inhibition effect under long-term exposure.

The present study was performed to evaluate the neurobehavioural deficit induced by nonylphenol (NP), a well-known xenobiotic chemical. The neurotoxic mechanism from oxidative stress and serotonin-related progress was also investigated. Caenorhabditis elegans was exposed at different levels of NP ranging from 0 to 200 μg L⁻¹ for 10 days. The results revealed that from a relatively low concentration (i.e., 10 μg L⁻¹), significant effects including decreased head thrashes, body bends and forging behaviour could be observed, along with impaired learning and memory behaviour plasticity. The level of reactive oxygen species (ROS) in head was significantly elevated with the increase of NP concentrations from 10 to 200 μg L⁻¹. Through antioxidant experiment, the oxidative damage caused by NP restored to some extent. At a NP concentration of 200 μg L⁻¹, the significant increased expression of stress-related genes, including sod-1, sod-3, ctl-2, ctl-3 and cyp-35A2 gene, was observed from integrated gene expression profiles. In addition, in comparison with wild-type N2 worms, the ROS accumulation was increased significantly with the mutation of sod-3. Tryptophan hydroxylase (TPH) in ADF and NSM neurons sharply decreased at the concentrations of 10–200 μg L⁻¹. The transcription of TPH synthesis-related genes and serotonin-related genes were both suppressed, including tph-1, cat-1, cat-4, ser-1, and mod-5. Overall, these results indicated that NP could induce neurotoxicity on Caenorhabditis elegans through excessive induction of ROS and disturbance synthesis of serotonin. The conducted research opened up new avenues for more effective exploration of neurotoxicity caused by NP.

Polycyclic aromatic hydrocarbons (PAHs), some of the most widespread organic contaminants, are highly toxic to soil microorganisms. Whether long-term polluted soils can still respond to the fresh input of pollutants is unknown. In this study, the soil enzyme activity, soil microbial community structure and function and microbial metabolism pathways were examined to systematically investigate the responses of soil microorganisms to fresh PAH stress. Microbial activity as determined by soil dehydrogenase and urease activity was inhibited upon microbe exposure to PAH stress. In addition, the soil microbial community and function were obviously shifted under PAH stress. Both microbial diversity and richness were decreased by PAH stress. Rhizobacter, Sphingobium, Mycobacterium, Massilia, Bacillus and Pseudarthrobacter were significantly affected by PAH stress and can be considered important indicators of PAH contamination in agricultural soils. Moreover, the majority of microbial metabolic function predicted to respond to PAH stress were affected adversely. Finally, soil metabolomics further revealed specific inhibition of soil metabolism pathways associated with fatty acids, carbohydrates and amino acids. Therefore, the soil metabolic composition distinctively changed, reflecting a change in the soil metabolism. In summary, fresh contaminant introduction into long-term polluted soils inhibited microbial activity and metabolism, which might profoundly affect the whole soil quality.

Despite substantial mitigation of particulate matter (PM) pollution during the past decade in Beijing, the response of aerosol chemistry to clean air action and meteorology remains less understood. Here we characterized the changes in aerosol composition as responses to emission reductions by using two-year long-term measurements in 2011/2012 and 2017/2018, and WRF-Chem model. Our results showed substantial decreases for all aerosol species except nitrate from 2011/2012 to 2017/2018. Chloride exhibited the largest decrease by 65–89% followed by organics (37–70%), mainly due to reductions in coal combustion emissions in winter and agriculture burning in June. Primary and secondary organic aerosol (SOA) showed comparable decreases by 61–70% in fall and winter, and 34–63% in spring and summer, suggesting that reductions in primary emissions might also suppress SOA formation. The changes in nitrate were negligible and even showed increases due to less reductions in NOₓ emissions and increased formation potential from N₂O₅ heterogeneous reactions. As a result, nitrate exceeded sulfate and became the major secondary inorganic aerosol species in PM with the contribution increasing from 14–21% to 22–32%. Further analysis indicated that the reductions in aerosol species from 2011/2012 to 2017/2018 were mainly caused by the decreases of severely polluted events (PM₁ > 100 μg m⁻³). WRF-Chem simulations suggested that the decreases in OA and sulfate in fall and winter were mainly resulted from emission reductions (27–36% and 25–43%) and favorable meteorology (4–10% and 19–30%), while they were dominantly contributed by emission changes in spring and summer. Comparatively, the changes in nitrate were mainly associated with meteorological variations while the contributions of emissions changes were relatively small. Our results highlight different chemical responses of aerosol species to emission changes and meteorology, suggesting that future mitigation of air pollution in China needs species-targeted control policy.

Rapid development in nanotechnology and incorporation of silver nanoparticles (AgNPs) in wide range of consumer products causing the considerable release of these NPs in the environment, leading concerns for ecosystem safety and plant health. In this study, rice (Oryza sativa) was exposed to AgNPs (0, 100, 200, 500 and 1000 mg L−1) in biochar amended (2 %w/v) and un-amended systems. Exposure of plants to AgNPs alone reduced the root and shoot length, biomass production, chlorophyll contents, photosynthesis related physiological parameters as well as macro-and micronutrients in a dose dependent manner. However, in case of biochar amendment, physiological parameters i.e., net photosynthesis rate, maximum photosynthesis rate, CO2 assimilation, dark respiration and stomatal conductance reduced only 16, 6, 7, 3 and 8%, respectively under AgNPs exposure at 1000 mg L−1 dose. Meanwhile, biochar at all exposure level of AgNPs decreased the bioaccumulation of Ag in rice root and shoot tissues, thus alleviated the phyto-toxic effects of NPs on plant growth. Moreover, results showed that biochar reduced the bioavailability of AgNPs by surface complexation, suppressing dissolution and release of toxic Ag+ ions in the growth medium. The presence of biochar at least decreased 2-fold tissue contents of Ag even at highest AgNPs (1000 mg L−1) concentration. These finding suggested that biochar derived from waste biomass resources can be used effectively to prevent the bioaccumulation and subsequent trophic level transfer of emerging Ag nano-pollutant in the environment.

Prenatal exposure to polychlorinated biphenyls (PCBs), persistent organic pollutants in food chains and environment, exerts negative effects on children's cognitive function. To study the long-term effects, we examined cognitive functions in the male children of women with substantial PCB exposure in Taiwan during 1978–1979 and investigated neural basis of cognitive function changes through structural magnetic resonance imaging (MRI) and functional MRI (fMRI), which included resting-state and task-activated fMRI with two paradigms: a 2-back task and a picture rotation task. Ten men aged 30.0 ± 3.7 years with prenatal exposure to PCBs and 11 unexposed controls aged 28.1 ± 3.1 years participated. Both groups had similar cognitive phenotypes and behavioral results. Structural MRI analysis results showed that the PCB group had increased cortical thickness over the right inferior parietal lobule. In the resting-state study, the PCB group showed alterations in the default mode network. During the tasks, the PCB group showed decreased task-induced deactivation signals in cognition–associated brain areas during the 2-back task but enhanced deactivations during the picture rotation task. This study demonstrated altered structural MRI as well as resting and task-related fMRI in men with prenatal PCB exposure, suggesting altered brain plasticity and compensatory neuropsychological performance.

There is increasing concern about phenolic environmental estrogens (PEEs) in river systems, especially in economically developed regions, because of their potential to impact ecological systems. We studied the distribution of, ecological risks from, and factors that influenced PEEs in the sediments from the Duliujian River in the Beijing-Tianjin-Hebei Urban Agglomeration and the Pearl River in Guangdong Province in China. The three target PEEs, nonyl phenol (4-NP), octyl phenol (4-t-OP), and bisphenol A (BPA), were detected in the sediments at concentrations ranging from 204.4 to 12604.3, 32.6 to 297.3, and from 12.8 to 298.4 ng g−1 in the Pearl River, and from 153.5 to 3614.9, 90.7 to 990.0, and 83.5–913.3 ng g−1 in the Duliujian River, respectively. The PEE concentrations were significantly and positively correlated with total organic carbon in the river sediments (p < 0.1). Urbanization influenced the distribution of PEEs and applications and discharges of PEEs were associated with large populations and industries. Rainfall and wastewater discharge patterns also influenced how PEEs were distributed in river sediments. The potential ecological risks from 4-NP, 4-t-OP, and BPA in these two rivers were high. Measures should be put in place to control the transport and storage of these compounds in river systems.