Per- and polyfluoroalkyl substances (PFASs) are environmentally and biologically persistent anthropogenic chemicals linked to adverse health outcomes. Epidemiological data have revealed association between exposure to specific PFAS and disruption of insulin level in bodies. However, the effect of PFASs on insulin secretion and the responsible molecular mechanism are poorly understood. In the present study, we used perfluorooctane sulfonate (PFOS) as a representative PFAS family member to investigate its effect on the insulin secretion in mouse pancreatic β cells (β-TC-6). Our results showed that exposure to PFOS inhibited silent information regulator 1 (SIRT1) activity, and molecular simulation showed PFOS could fit into the pocket overlapped with the nicotinamide adenine dinucleotide (NAD⁺) binding cavity in SIRT1. PFOS exposure upregulated uncoupling protein 2 (UCP2) expression, and this upregulation was blunted in the presence of Ex-527, a SIRT1 specific inhibitor. The mitochondria membrane potential (ΔΨm), as well as the glucose-induced ATP production and Ca²⁺ influx decreased under PFOS treatment. PFOS continual exposure (48 h) impaired glucose stimulated insulin secretion (GSIS), while the gene expression of insulin was not significantly altered. Importantly, the SIRT1 activator and UCP2 inhibitor could partly reverse the PFOS-induced impairment of GSIS. Taken together, the results suggested that PFOS continual exposure could inhibit SIRT1 activity, and the SIRT1-UCP2 pathway mediated, at least partially, the PFOS induced GSIS impairment.

In Lebanon, previous studies have indicated an onset of cardiovascular diseases 12 years earlier than in other parts of the world, suggesting the presence of additional risk factors specific to Lebanon. Measurements of airborne particles in Lebanon surpass the recommendations of the World Health Organization by over 150%. This study examined the association between obstructive coronary artery disease (CAD), assessed by a novel marker calculated from coronary catheterization, and markers of air pollution, specifically polycyclic aromatic hydrocarbons (PAHs), in a cohort of 258 patients seen at the American University of Beirut Medical Center since 2014. The concentrations of four types of hydroxylated polycyclic aromatic hydrocarbons (OHPAHs), 2-OHNAP, 2-OHFLU, 3-OHPHE, and 1-OHPYR, were measured in the urine samples of these patients using high performance liquid chromatography coupled with fluorescence detector. Results showed that the OHPAH concentrations were higher than what was reported in high-income countries and, most notably, the levels for non-smokers in this study were higher than those of smokers and some occupational workers in other countries. This implies that patients were exposed to high levels of PAHs, which originate from combustion sources. In particular, 1-OHPYR showed a significant association with presence of obstructive CAD, even after adjusting for covariates like age, sex, and diabetes. Smokers or not, this association has implications for public health and calls for urgent need to pass regulations to reduce the emissions of PAH sources, such as cars, diesel generators, and incinerators.

Seabirds are apex predators in the marine environment and well-known ecosystem engineers, capable of changing their terrestrial habitats by introducing marine-derived nutrients via deposition of guano and other allochthonous inputs. However, with the health of the world’s oceans under threat due to anthropogenic pressures such as organic, inorganic, and physical pollutants, seabirds are depositing these same pollutants wherever they come to land. Using data from 2018 to 2020, we quantify how the Flesh-footed Shearwater (Ardenna carneipes) has inadvertently introduced physical pollutants to their colonies on Lord Howe Island, a UNESCO World Heritage site in the Tasman Sea and their largest breeding colony, through a mix of regurgitated pellet (bolus) deposition and carcasses containing plastic debris. The density of plastics within the shearwater colonies ranged between 1.32 and 3.66 pieces/m² (mean ± SE: 2.18 ± 0.32), and a total of 688,480 (95% CI: 582,409–800,877) pieces are deposited on the island each year. Our research demonstrates that seabirds are a transfer mechanism for marine-derived plastics, reintroducing items back into the terrestrial environment, thus making seabird colonies a sink for plastic debris. This phenomenon is likely occurring in seabird colonies across the globe and will increase in severity as global plastic production and marine plastic pollution accelerates without adequate mitigation strategies.

The coronavirus disease (COVID-19) has become a global public health threaten. A series of strict prevention and control measures were implemented in China, contributing to the improvement of air quality. In this study, we described the trend of air pollutant concentrations and the incidence of COVID-19 during the epidemic and applied generalized additive models (GAMs) to assess the association between short-term exposure to air pollution and daily confirmed cases of COVID-19 in 235 Chinese cities. Disease progression based on both onset and report dates as well as control measures as potential confounding were considered in the analyses. We found that stringent prevention and control measures intending to mitigate the spread of COVID-19, contributed to a significant decline in the concentrations of air pollutants except ozone (O₃). Significant positive associations of short-term exposure to air pollutants, including particulate matter with diameters ≤2.5 μm (PM₂.₅), particulate matter with diameters ≤10 μm (PM₁₀), and nitrogen dioxide (NO₂) with daily new confirmed cases were observed during the epidemic. Per interquartile range (IQR) increase in PM₂.₅ (lag0-15), PM₁₀ (lag0-15), and NO₂ (lag0-20) were associated with a 7% [95% confidence interval (CI): (4–9)], 6% [95% CI: (3–8)], and 19% [95% CI: (13–24)] increase in the counts of daily onset cases, respectively. Our results suggest that there is a statistically significant association between ambient air pollution and the spread of COVID-19. Thus, the quarantine measures can not only cut off the transmission of virus, but also retard the spread by improving ambient air quality, which might provide implications for the prevention and control of COVID-19.

Aquaculture impacts on aquatic organic matter and ecosystem integrity are poorly understood, especially in tropical regions. Here, we investigated the impacts of Nile tilapia net cage farming on the elemental stoichiometry, fluorescence components, and stable isotopes of dissolved organic matter (DOM) of the large, tropical Furnas Reservoir (SE Brazil). Early-stage fish farming, i.e., relatively small and recently implemented farms, had detectable incipient effects on DOM characteristics, and these effects differed between reservoir branches. In the less eutrophic Rio Grande branch of the reservoir, we found a reduction in natural humic-like DOM components and an increase in a protein-like DOM component as far as 100 m away from fish farms. Further, we observed a decrease in δ¹⁵N-TDN due to fish farming. In the more eutrophic Rio Sapucaí branch, there were only local decreases in C:N ratios, as well as rises in C:P and N:P of DOM due to fish farming. These results suggest that early-stage fish farming had local but detectable effects on aquatic DOM that depended on previous eutrophication levels and highlight the need to assess the early impacts of fish farming on tropical reservoirs by combining different monitoring strategies.

Diethyl phthalate (DEP), as a kind of universally used plasticizer, has aroused considerable public concern owing to its wide detection, environmental stability, and potential health risks. In this work, the highly efficient removal of DEP by ferrate (VI) (Fe(VI)) was systematically explored in soil environment. The effects of the oxidant dosages, soil types, as well as the presence of coexisting cations and anions in tested soil on DEP removal were evaluated. When the dosage of Fe(VI) was 20 mM, complete removal of DEP (50 μg/g) was achieved in the tested soil after 2 min of reaction. Furthermore, the removal rate of DEP was closely related to the soil types, and the degradation rates were decreased obviously in red soil (RS), black soil (BS) and paddy soil (PS), probably due to the acidic condition and high content of organic matters. Moreover, the presence of Ca²⁺, Mg²⁺ and Al³⁺ in soil can inhibit the removal of DEP by Fe(VI), while SO₄²⁻ has an slightly promotion effect. Six oxidation intermediates were detected in the reaction process of DEP, product analysis revealed that the transformation of DEP was mainly through two pathways, including hydrolysis and hydroxylation reactions, which were probably mediated by oxygen atom transfer process of Fe(VI). Based on the frontier electron density theory calculation, two ester groups of DEP were prone to be attacked by Fe(VI), and the hydroxyl addition tended to occur at the para-position of one of the ester groups on the benzene ring. This study provides a novel approach for phthalate esters removal from soil using Fe(VI) oxidation and shows new insights into the oxidation mechanisms.

Pesticides are heavily applied in rice–vegetable rotations in tropical China, yet publicly available information on the contamination and risk of currently used pesticides (CUPs) and legacy pesticides (LPs) in surface waters of river basins draining these areas is very limited. Therefore, in two tropical river basins (Nandu River and Wanquan River basins) dominated by rice–vegetable rotations in Hainan, China, pesticides were analyzed in 256 surface water samples in wet and dry seasons. Forty-one pesticides were detected, and total concentrations ranged from not detectable to 24.2 μg/L. Carbendazim and imidacloprid were the two most prevalent CUPs, detected in 59.8% and 17.7%, respectively, of surface water samples at concentrations above 0.1 μg/L. Chlorpyrifos was the main LP, detected in 9.0% of samples at a concentration above 0.05 μg/L. The fungicides difenoconazole and emamectin benzoate, the herbicide butachlor, and the insecticide acetamiprid occurred in ≥12.5% samples at concentrations above 0.1 μg/L. Surface waters typically (85.2%) contained 5 to 15 residues, with an average of nine. Seasonally, the concentrations of the 41 pesticides were in the order January > July > November > September. Spatially, the composition of the main CUPs (not LPs) was significantly different depending on position in the drainage, which also changed with seasons. Crop and pest types and wet and dry seasons were the key factors controlling the spatiotemporal distribution of CUPs and LPs in surface waters. On the basis of evaluations of the exposures to individual pesticides and the dominant combinations with ≥8 pesticides, multiple pesticides were likely a significant risk to aquatic organisms, although noncarcinogenic and carcinogenic risks to humans were low. This study provides valuable data to better understand pesticide occurrence and ecological risks in river basins draining areas with rice–vegetable rotation systems in tropical China.

Cadmium (Cd) and lead (Pb) pollution in soil and their accumulation in edible parts possess a worldwide eco-environmental and health risk, especially in developing countries. Recently, organosilicone fertilizer (OSiF) has been reported to reduce uptake of heavy metals, but the effectiveness has not been verified and its associated mechanisms are not fully understood. This work investigated whether and how OSiF and mineral silicon fertilizer (MSiF) affect mitigation of Cd and Pb stress in rice (Oryza sativa). Both soil incubation and pot experiments were conducted to assess the effect of OSiF and MSiF on bioavailability of Cd and Pb in soil and their accumulation in rice. Additionally, a hydroponic experiment was conducted to study whether Si in rice can alleviate Cd stress. We found that both Si fertilizers could increase soil pH, induce the transformation of the acid soluble and reducible fractions of Cd and Pb to the oxidizable and residual fractions in soil, decreasing their bioavailability and the uptake of Cd and Pb in rice. However, Si in OSiF was not phyto-available, but Si in MSiF was available since available Si in soil and Si in plant increased in MSiF treatments but not in OSiF treatments. Meanwhile, rice grain yields significantly increased and the Cd and Pb content of brown rice reduced in MSiF treatments but not in OSiF treatments. In addition, Si was found to be able to alleviate Cd stress by improving the antioxidant capacity of rice. These results suggested that the decreased Cd and Pb accumulation in OSiF-treated rice was due to Cd and Pb immobilization in soil simply with pH increase, but in MSiF-treated rice Cd and Pb immobilization in soil (ex planta effect) and Si-conferred inhibitory effect of root-to-shoot Cd and Pb transport (in planta effect) contribute to the lower accumulation in rice.

Exploring effective uses of waste concrete powder (WCP), produced from recycling of construction & demolition waste is beneficial to the environment and sustainable development. In this study, WCP was first treated thermally to enhance the ability to remove Pb (II) from aqueous solutions. The experimental results revealed that the thermal treatment could enhance adsorption capacity due to modification of calcium bonding and pore structure of WCP. Preparation parameters such as temperature, particle size, and water-cement ratio were investigated to obtain the optimal operational conditions. Batch adsorption experiments were performed to explore influence factors of pH (1.00–6.00), ionic strength (0.05–2 mol/L), dosage (2–50 g/L), and temperature (25–45 °C). The pseudo-second-order kinetics model could adequately describe the adsorption process, and the Langmuir model was capable to predict the isotherm data well in the low concentration region (C₀ < 500 mg/L). The maximum uptake capacity for Pb (II) calculated by Langmuir model at 25, 35 and 45 °C were 46.02, 38.58 and 30.01 mg/g respectively, and the removal rate of Pb (II) was 92.96% at a dosage of 50 g/L (C₀ = 1000 mg/L). Precipitation, ion exchange, and surface complexation were identified to be the main mechanisms of Pb (II) adsorption through microscopic investigation by SEM-EDX, XRD, FTIR, XPS, and BET inspections. The study confirms that the WCP after thermal modification, can be selected as a promising adsorbent for the high performance and eco-friendliness.

Exposure to traffic-related air pollution (TRAP) may enhance the risk of cardiovascular disease. However, the short-term effects of TRAP components on the cardiovascular system are not well understood. We conducted a randomized, double-blinded, crossover intervention study in which 39 healthy university students spent 2 h next to a busy road. Participants wore a powered air-purifying respirator (PAPR) or an N95 mask. PAPRs were equipped with a filter for particulate matter (PM), a PM and volatile organic compounds (VOCs) filter or a sham filter. Participants were blinded to PAPR filter type and underwent randomized exposures four times, once for each intervention mode. Blood pressure (BP), heart rate (HR) and heart rate variability (HRV) were measured before, during and for 6 h after the roadside exposure. Linear mixed-effect models were used to evaluate the effects of the interventions relative to baseline controlling for other covariates. All HRV measures increased during and following exposure for all intervention modes. Some HRV measures (SDNN and rMSSD during exposure and SDNN after exposure) were marginally affected by PM filtration. Wearing the N95 mask affected VLF power and rMSSD responses to traffic exposure differently than the PAPR interventions. Both systolic and diastolic BP increased slightly during exposure, but then were generally lower than baseline after exposure for the sham and filter interventions. HR, which fell during exposure and mostly remained lower than baseline after exposure, was lower yet with all filter interventions compared to the sham mode following exposure. Therefore, short-term exposure to traffic acutely affects HRV, BP and HR, but N95 mask and PAPR interventions generally show little efficacy in reducing these effects. Removing the PM component of TRAP has some limited effects on HRV responses to exposure but exaggerates the traffic-related decrease in HR. HRV findings from N95 mask interventions need to be interpreted cautiously.