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Результаты 601-610 из 7,921
Triphenyl phosphate delayed pubertal timing and induced decline of ovarian reserve in mice as an estrogen receptor antagonist
2021
Ma, Haojia | Ishida, Keishi | Xu, Chenke | Takahashi, Kyosuke | Li, Yu | Zhang, Chenhao | Kang, Qiyue | Jia, Yingting | Hu, Wenxin | Matsumaru, Daisuke | Nakanishi, Tsuyoshi | Hu, Jianying
Although concerns have been raised about the adverse effects of triphenyl phosphate (TPhP) on female fertility, its risk to ovarian functioning remains unknown. In this study, female C57BL/6 mice at postnatal day 21 were exposed on a daily basis to TPhP dose of 2, 10, and 50 mg/kg for 40 days. A significant delay in pubertal timing was observed in the mice exposed to 50 mg/kg of TPhP. An estrogen-responsive reporter transgenic mice assay demonstrated that TPhP significantly downregulated the estrogen receptor (ER) signaling by 45.1% in the whole body in the 50 mg/kg group, and by 14.7–43.7% in the uterus for all exposure groups compared with the control. This strong antagonistic activity of TPhP toward ER explained the delay in pubertal timing. A significant reduction in the number of follicles in all stages was observed in mice after being exposed to TPhP for 40 days at concentrations of 10 and 50 mg/kg, resulting in a decline of the ovarian reserve. The elevation of the follicle-stimulating hormone concentration may have contributed to this phenomenon, as controlled by the antagonistic activity of TPhP toward ER in the brain. The toxic effects of TPhP on ovarian functioning highlight this chemical as a potential risk factor for female fertility.
Показать больше [+] Меньше [-]Ecological implications beyond the ecotoxicity of plastic debris on marine phytoplankton assemblage structure and functioning
2021
Casabianca, Silvia | Bellingeri, Arianna | Capellacci, Samuela | Sbrana, Alice | Russo, Tommaso | Corsi, Ilaria | Penna, Antonella
Plastic pollution is a global issue posing a threat to marine biota with ecological implications on ecosystem functioning. Micro and nanoplastic impact on phytoplankton autotrophic species (e.g., cell growth inhibition, decrease in chlorophyll a and photosynthetic efficiency and hetero-aggregates formation) have been largely documented. However, the heterogeneity of data makes rather difficult a comparison based on size (i.e. micro vs nano). In addition, knowledge gaps on the ecological impact on phytoplankton assemblage structure and functioning are evident. A new virtual meta-analysis on cause-effect relationships of micro and nanoplastics on phytoplankton species revealed the significant effect posed by polymer type on reducing cell density for tested PVC, PS and PE plastics. Linked with autotrophic phytoplankton role in atmospheric CO₂ fixation, a potential impact of plastics on marine carbon pump is discussed. The understanding of the effects of microplastics and nanoplastics on the phytoplankton functioning is fundamental to raise awareness on the overall impact on the first level of marine food web. Interactions between micro and nanoplastics and phytoplankton assemblages have been quite documented by in vitro examinations; but, further studies considering natural plankton assemblages and/or large mesocosm experiments should be performed to evaluate and try predicting ecological impacts on primary producers.
Показать больше [+] Меньше [-]Exposure to nanoparticles derived from diesel particulate filter equipped engine increases vulnerability to arrhythmia in rat hearts
2021
Rossi, Stefano | Buccarello, Andrea | Caffarra Malvezzi, Cristina | Pinelli, Silvana | Alinovi, Rossella | Guerrero Gerboles, Amparo | Rozzi, Giacomo | Leonardi, Fabio | Bollati, Valentina | De Palma, Giuseppe | Lagonegro, Paola | Rossi, F. (Francesca) | Lottici, Pier Paolo | Poli, Diana | Statello, Rosario | Macchi, Emilio | Miragoli, Michele
Air pollution is well recognized as a central player in cardiovascular disease. Exhaust particulate from diesel engines (DEP) is rich in nanoparticles and may contribute to the health effects of particulate matter in the environment. Moreover, diesel soot emitted by modern engines denotes defective surfaces alongside chemically-reactive sites increasing soot cytotoxicity. We recently demonstrated that engineered nanoparticles can cross the air/blood barrier and are capable to reach the heart. We hypothesize that DEP nanoparticles are pro-arrhythmogenic by direct interaction with cardiac cells. We evaluated the internalization kinetics and the effects of DEP, collected from Euro III (DEPe3, in the absence of Diesel Particulate Filter, DPF) and Euro IV (DEPe4, in the presence of DPF) engines, on alveolar and cardiac cell lines and on in situ rat hearts following DEP tracheal instillation. We observed significant differences in DEP size, metal and organic compositions derived from both engines. DEPe4 comprised ultrafine particles (<100 nm) and denoted a more pronounced toxicological outcome compared to DEPe3. In cardiomyocytes, particle internalization is fastened for DEPe4 compared to DEPe3. The in-vivo epicardial recording shows significant alteration of EGs parameters in both groups. However, the DEPe4-instilled group showed, compared to DEPe3, a significant increment of the effective refractory period, cardiac conduction velocity, and likelihood of arrhythmic events, with a significant increment of membrane lipid peroxidation but no increment in inflammation biomarkers. Our data suggest that DEPe4, possibly due to ultrafine nanoparticles, is rapidly internalized by cardiomyocytes resulting in an acute susceptibility to cardiac electrical disorder and arrhythmias that could accrue from cellular toxicity. Since the postulated transfer of nanoparticles from the lung to myocardial cells has not been investigated it remains open whether the effects on the cardiovascular function are the result of lung inflammatory reactions or due to particles that have reached the heart.
Показать больше [+] Меньше [-]Controlled treatment of a high velocity anisotropic aquifer model contaminated by hexachlorocyclohexanes
2021
Bouzid, Iheb | Maire, Julien | Laurent, Fabien | Broquaire, Mathias | Fatin-Rouge, Nicolas
Xanthan gels were assessed to control the reductive dechlorination of hexachlorocyclohexanes (HCHs) and trichlorobenzenes (TCBs) in a strong permeability contrast and high velocity sedimentary aquifer. An alkaline degradation was selected because of the low cost of NaOH and Ca(OH)₂. The rheology of alkaline xanthan gels and their ability to deliver alkalinity homogeneously, while maintaining the latter, were studied. Whereas the xanthan gels behaved like non-Newtonian shear-thinning fluids, alkalinity and Ca(OH)₂ microparticles had detrimental effects, yet, the latter decreased with the shear-rate. Breakthrough curves for the NaOH and Ca(OH)₂ in xanthan solutions, carried out in the lowest permeability soil (9.9 μm²), demonstrated the excellent transmission of alkalinity, while moderate pressure gradients were applied. Injection velocities ranging from 1.8 to 3.8 m h⁻¹ are anticipated in the field, given the permeability range from 9.9 to 848.7 μm². Despite a permeability contrast of 8.7 in an anisotropic aquifer model, the NaOH and the Ca(OH)₂ both in xanthan gels spread only 5- and 7-times faster in the higher permeability zone, demonstrating that the delivery was enhanced. Moreover, the alkaline gels which were injected into a high permeability layer under lateral water flow, showed a persistent blocking effect and longevity (timescale of weeks), in contrast to the alkaline solution in absence of xanthan. Kinetics of alkaline dechlorination carried out on the historically contaminated soil, using the Ca(OH)₂ suspension in xanthan solution, showed that HCHs were converted in TCBs by dehydrodechlorination, whereas the latter were then degraded by reductive hydrogenolysis. Degradation kinetics were achieved within 30 h for the major and most reactive fraction of HCHs.
Показать больше [+] Меньше [-]Effects of plastic particles on germination and growth of soybean (Glycine max) : A pot experiment under field condition
2021
Li, Bintao | Huang, Shan | Wang, Haoming | Liu, Mengjuan | Xue, Sha | Tang, Darrell | Cheng, Wanli | Fan, Tinglu | Yang, Xiaomei
Plastic residues have become a serious environmental problem in areas where agricultural plastic film are used intensively. Although numerous of studies have been done to assess its impacts on soil quality and crop yields, the understanding of meso-plastic particles effects on plant is still limited. In this study, low density polyethylene (PE) and biodegradable plastic (Bio) mulch film were selected to study the effects of meso-plastic debris on soybean germination and plant growth with the accumulation levels of 0%, 0.1%, 0.5% and 1% in soil (w: w, size ranging 0.5–2 cm) by a pot experiment under field condition. Results showed that the germination viability of soybean seeds was reduced to 82.39%, 39.44% and 26.06% in the treatments with 0.1%, 0.5% and 1% added plastic debris compared to the control (CK), respectively, suggesting that plastic residues in soil inhibit the viability of soybean seed germination. The plastic debris had a significant negative effect on plant height and culm diameter during the entire growth stage of soybean. Similarly, the leaf area at harvest was reduced by 1.97%, 6.86% and 11.53% compared to the CK in the treatments with 0.1%, 0.5% and 1% plastic debris addition, respectively. In addition, the total plant biomass under plastic addition was reduced in both the flowering and harvesting stages, compared to the CK. For the different type of plastic residues, plant height, leaf area and root/shoot ratio at group PE were significantly lower than those of groups treated by Bio. In conclusion, PE debris had a greater negative effects on plant height, culm diameter, leaf area and root/shoot ratio while Bio debris mainly showed the adverse effects on germination viability and root biomass especially at the flowering stage. Therefore, further research is required to elaborate plastic particles’ effects on different stages of crops and soil quality.
Показать больше [+] Меньше [-]Interannual and seasonal variabilities in soil NO fluxes from a rainfed maize field in the Northeast China
2021
Su, Chenxia | Zhu, Weixing | Kang, Ronghua | Quan, Zhi | Liu, Dongwei | Huang, Wentao | Shi, Yi | Chen, Xin | Fang, Yunting
Nitric oxide (NO) plays a critical role in atmospheric chemistry and also is a precursor of nitrate, which affects particle matter formation and nitrogen deposition. Agricultural soil has been recognized as a main source of atmospheric NO. However, quantifying the NO fluxes emitted from croplands remains a challenge and in situ long-term measurements of NO are still limited. In this study, we used an automated sampling system to measure NO fluxes with a high temporal resolution over two years (April 2017 to March 2019) from a rainfed maize field in the Northeast China. The cumulative annual NO emissions were 8.9 and 2.3 kg N ha⁻¹ in year 1 (April 2017 to March 2018) and year 2 (April 2018 to March 2019), respectively. These interannual differences were largely related to different weather conditions encountered. In year 1, a month-long drought before and after the seeding and fertilizing reduced plant N uptake and dramatically increased soil N concentration. The following moderate rainfalls promoted large amount of NO emissions, which remained high until late September. The NO fluxes in both years showed clearer seasonal patterns, being highest after fertilizer application in summer, and lowest in winter. The seasonal patterns of NO fluxes were mainly controlled by soil available N concentrations and soil temperatures. The contribution of NO fluxes during the spring freeze-thaw in both years was no more than 0.2% of the annual NO budget, indicating that the freeze-thaw effect on agricultural NO emissions was minimal. In addition, with high-resolution monitoring, we found that soil not only act as a NO source but also a sink. Long-term and high-resolution measurements help us better understand the diurnal, seasonal, and annual dynamics of NO emissions, build more accurate models and better estimate global NO budget and develop more effective policy responses to global climate change.
Показать больше [+] Меньше [-]Thiol-functionalized nano-silica for in-situ remediation of Pb, Cd, Cu contaminated soils and improving soil environment
2021
Lian, Mingming | Wang, Longfei | Feng, Qiaoqiao | Niu, Liyong | Zhao, Zongsheng | Wang, Pengtao | Song, Chunpeng | Li, Xiaohong | Zhang, Zhijun
Heavy metal contamination has been threatening the health of human beings. To decrease the bio-toxicity of heavy metals, a thiol-functionalized nano-silica (SiO₂-SH) was adopted to remediate the soil contaminated by lead (Pb), cadmium (Cd) and copper (Cu). The remediation effect of SiO₂-SH on contaminated soils was investigated by the uptake of the heavy metals into lettuce and pakchoi in pot experiment. The bio-toxicity of the SiO₂-SH was evaluated, and its immobilization mechanisms were proposed by the fraction distribution of Cd, Pb and Cu. It was found that the SiO₂-SH can significantly reduce the uptake of Cd, Pb, Cu into pakchoi by 92.02%, 68.03%, 76.34% and into lettuce by 89.81%, 43.41%, 5.76%, respectively. The chemical species analyses of Cd, Pb, Cu indicate SiO₂-SH can transform the heavy metal in acid soluble states into reducible fraction and oxidizable fraction, thereby inhibiting the extraction of heavy metals into soil solution. The concentrations of microbial biomass carbon, organic matter, and cation exchange capacity of the soil increased while the soil bulk density decreased after remediation. Those changes demonstrate that SiO₂-SH not only has no bio-toxic impact on the soil environment but also improves the soil environment, which proves the prepared SiO₂-SH is environmental-friendly. The SiO₂-SH could be a promising amendment for heavy metal contaminated soils.
Показать больше [+] Меньше [-]Sustainable functionalized metal-organic framework NH2-MIL-101(Al) for CO2 separation under cryogenic conditions
2021
Bābar, Muḥammad | Mubashir, Muhammad | Mukhtar, Ahmad | Saqib, Sidra | Ullah, Sami | Bustam, Mohamad Azmi | Show, Pau Loke
In this study, a sustainable NH₂-MIL-101(Al) is synthesized and subjected to characterization for cryogenic CO₂ adsorption, isotherms, and thermodynamic study. The morphology revealed a highly porous surface. The XRD showed that NH₂-MIL-101(Al) was crystalline. The NH₂-MIL-101(Al) decomposes at a temperature (>500 °C) indicating excellent thermal stability. The BET investigation revealed the specific surface area of 2530 m²/g and the pore volume of 1.32 cm³/g. The CO₂ adsorption capacity was found to be 9.55 wt% to 2.31 wt% within the investigated temperature range. The isotherms revealed the availability of adsorption sites with favorable adsorption at lower temperatures indicating the thermodynamically controlled process. The thermodynamics showed that the process is non-spontaneous, endothermic, with fewer disorders, chemisorption. Finally, the breakthrough time of NH₂-MIL-101(Al) is 31.25% more than spherical glass beads. The CO₂ captured by the particles was 2.29 kg m⁻³. The CO₂ capture using glass packing was 121% less than NH₂-MIL-101(Al) under similar conditions of temperature and pressure.
Показать больше [+] Меньше [-]Remediation of artificially contaminated soil and groundwater with copper using hydroxyapatite/calcium silicate hydrate recovered from phosphorus-rich wastewater
2021
Liu, Yiyang | Zhang, Rongbin | Sun, Zhenjie | Shen, Qin | Li, Yuan | Wang, Yuan | Xia, Siqing | Zhao, Jianfu | Wang, Xuejiang
Excessive copper (Cu) in contaminated soil and groundwater has attracted continuous attentions due to the bioaccumulation and durability. In this study, the feasibility of remediation of heavy metal pollution in soil and groundwater was investigated using hydroxyapatite/calcium silicate hydrate (HAP/C–S–H) recovered from phosphorus-rich wastewater in farmland. The results show that the pH has a strong effect on copper removal from Cu-contaminated groundwater but the impact of ion strength on the removal is weak. In general, high pH and low ion strength give better results in copper removal. Kinetic and isotherm data from the study fit well with Pseudo-second-order kinetic model and Langmuir isotherm model, respectively. The maximum adsorption capacity of HAP/C–S–H (138 mg/g) was higher than that of C–S–H (90.3 mg/g) when pH value, temperature, and ionic strength were 5, 308 K, and 0.01 M, respectively. Thermodynamics results indicate that Cu removal is a spontaneous and endothermic process. X-ray diffraction (XRD) results show that the mechanism of copper removal involves physical adsorption, chemical precipitation and ion exchange. For the remediation of Cu-contaminated soil, 76.3% of leachable copper was immobilized by HAP/C–S–H after 28 d. Acid soluble Cu, the main contributor to biotoxicity, decreased significantly while reducible and residual Cu increased. After immobilization, the acid neutralization capacity of the soil increased and the dissolution of copper was substantially reduced in near-neutral pH. It can be concluded that HAP/C–S–H is an effective, low-cost and eco-friendly reagent for in-situ remediation of heavy metal polluted soil and groundwater.
Показать больше [+] Меньше [-]Dichlorodiphenyltrichloroethane metabolites inhibit DNMT1 activity which confers methylation-specific modulation of the sex determination pathway
2021
Hu, Junjie | Yang, Yan | Lv, Xiaomei | Lao, Zhilang | Yu, Lili
Dichlorodiphenyltrichloroethane (DDT) poses a significant health risk to humans which is associated with genomic DNA hypomethylation. However, the mechanism and biological consequences remain poorly understood. In vitro assays confirmed that the DDT metabolites 2,2-bis(p-chlorophenyl)-acetic acid (DDA) and 1-chloro-2,2-bis-(p-chlorophenyl)ethylene (DDMU), but not other DDT metabolites, significantly inhibited DNA methyltransferase 1 (DNMT1) activity, leading to genomic hypomethylation in cell culture assays. DNMT1 as a target for DNA hypomethylation induced by DDT metabolites was also confirmed using cell cultures in which DNMT1 was silenced or highly expressed. DDA and DDMU can modify methylation markers in the promoter regions of sexual development-related genes, and change the expression of Sox9 and Oct4 in embryonic stem cells. Molecular docking indicated that DDA and DDMU bound to DNMT1 with high binding affinity. Molecular dynamic simulation revealed that DDA and DDMU acted as allosteric modulators that reshaped the conformation of the catalytic domain of DNMT1. These findings provide a new insight into DDT-induced abnormalities in sexual development and demonstrate that selective binding to DNMT1 by DDA and DDMU can interfere with human DNMT1 activity and regulate the expression of the Sox9 and Oct4 genes.
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