Keeping in view the expanding environmental pollution and irrigation water deficit, a pot experiment was performed for the upland (Huyou2, Hanyou737) and paddy rice cultivars (Taigeng8; Yixiang2292), to study soil liming effects on methane (CH₄) and nitrous oxide (N₂O) emission, bioavailability and accumulation of Cd, Pb in upland and paddy rice. Upland rice reduced 90% of soil CH₄ emission as compared to paddy conditions. Soil CH₄ emission decreased by 45% and 39% with dolomite, and it reduced by 35% and 33% with lime treatment both in upland and paddy conditions, respectively. Soil N₂O emission decreased by 44% and 52% with dolomite, and with the lime application, it was reduced by 37% and 44% for both upland and paddy conditions respectively. Reduction in soil DTPA-extractable Cd was between 37-53% and 43–80% with dolomite and 16–37% and 24–72% Cd decreased with lime application in upland and paddy conditions respectively. Soil DTPA-extractable Pb reduced by 27–44% and 25–53% with dolomite and 16–40% and 11–42% with soil-applied lime in upland and paddy conditions, respectively. Cd accumulation in rice grain was decreased by 47–88% and 62–79% with dolomite and 31–86% and 45–52% reduction by lime application in upland and paddy rice respectively. Rice grain Pb reduced by 58–91% and 66–78% with dolomite application and 32–71% and 44–71% with lime in upland and paddy rice, respectively. Our results showed that soil liming significantly reduced soil N₂O and CH₄ emission and Cd, Pb accumulation in rice grain, but dolomite was more effective as compared to lime. Altogether, results of this study suggest that upland rice can be cultivated in CdPb polluted soils with least soil CH₄ emission. Cd and Pb toxicity, accumulation, and N₂O emission in upland rice can be minimized by soil liming of 3 g kg⁻¹ and optimizing the nutrients composition of the soil.

Given factors such as their persistence and toxicity, legacy brominated flame retardants (BFRs) like polybrominated diphenyl ethers (PBDEs) and hexabromocyclododecane (HBCDD), are designated as persistent organic pollutants (POPs) and are subject to regulation. Waste streams likely represent a substantial reservoir of legacy BFRs given that they were once widely applied to goods which are increasingly likely to be obsolete. Waste streams are also increasingly likely to be a source of emerging flame retardants, in particular, novel BFRs (NBFRs), the halogenated norbornene flame retardant Dechlorane Plus (DDC-CO) and the brominated, chlorinated or non-halogenated organophosphate triester flame retardants (PFRs). Many bird populations rely on landfill and its surrounding land-use for inter alia the opportunities it provides for activities such as foraging and resting. However, studies on captive and wild (free-living) birds have demonstrated deleterious effects of several FRs. Globally, approximately 250 bird species, including many of conservation concern, are reported to use landfill and surrounding habitat (including wastewater treatment operations), thus putting birds potentially at risk of exposure to such chemicals. We synthesise and critically evaluate a total of 18 studies covering eight avian species published between 2008 and 2018 (inclusive) across four continents that report flame retardant (FR) burdens in birds utilising landfill. Several such studies found FRs at among the highest concentrations detected in wild biota to date. We recommend that ongoing research be focused on landfill-associated birds, given that landfill is an important source of FRs and other anthropogenic chemicals, and particularly at sites where species are of conservation concern. We suggest ways in which the comparative power of studies could be enhanced in the future, the reporting of a minimum common suite of key chemicals, and where feasible, standardisation of the tissue compartments (i.e., eggs) to be studied. We conclude by identifying future research directions.

Employing biochar for environmental remediation has been widely practiced. Nonetheless, the reduction mechanisms of hexavalent chromium (Cr(VI)) in the presence of biochar have not been fully elucidated (i.e., direct or indirect reduction of Cr(VI) by biochar). In particular, the effect of light on Cr(VI) reduction by biochar was rarely reported. Thus, to clarify the reduction mechanisms of Cr(VI) by biochar at the fundamental level, this study laid great emphasis on the photo-induced reduction of Cr(VI) in the application of biochar. Biochar releases dissolved organic matter (DOM), the DOM can extract Fe(III) from soil by complexation, and the complexes can be photo-reacted under the light. In these respects, Fe(II) formed by the photo-induced reaction of DOM-Fe(III) was particularly evaluated in this study. To evaluate that, three biomass samples (rice straw, granular sludge from an up-flow anaerobic sludge blanket, and spent coffee ground) were torrefied to biochar. To circumvent the adsorption of Cr(VI) onto biochar, biochar extractives (served as a source for DOM) and Fe(III) solution were tested with/without UV light to prove Fe(II) formation. This study experimentally proved that the more Fe(II) under the UV radiation was formed in the co-existence with biochar extractives and Fe(III). All experimental data from three biochar samples were indeed very similar. Cr(VI) reduction by Fe(II) from GB, RB, and CB reached up to 96, 79, and 100%, respectively. The different reduction efficiency signified that the low molecular weight of organic acids, such as oxalate, were more sensitive to the UV light, thereby resulting in the enhanced Fe(II) formation. Such Fe(II) formation subsequently led to the high reduction efficiency of Cr(VI).

Juvenile hormone analogue (JHA) insecticides are endocrine disrupters that interfere with hormonal action in insects by mimicking their juvenile hormones (JH). As the structure and functions of methyl farnesoate in crustaceans are similar to those of JH in insects, exogenous JHA insecticides could have adverse effects on the development and reproduction of crustaceans. This study examined the toxic effects of two JHA insecticides, fenoxycarb and methoprene, on a freshwater shrimp model of cherry shrimp, Neocaridina davidi. Both insecticides had detrimental effects on cherry shrimp, but fenoxycarb was more toxic than methoprene. Chronic exposure to these insecticides reduced the shrimp's body length and molting frequency. Based on transcriptome annotations for N. davidi, we identified important gene homologues that were active in both insect JH biosynthetic and degradative pathways as well as JH and ecdysteroid signaling pathways. Chronic treatments with JHAs had significant effects on these genes in N. davidi. Our transcriptomic analysis showed that genes involved in the pathways related to cuticle development, serine protease activity, and carbohydrate, peptide and lipid metabolic processes were differentially expressed in shrimp exposed to JHAs. These results demonstrate the toxicity of fenoxycarb and methoprene to freshwater crustaceans and indicate the need to monitor the use of JHA insecticides.

Microplastics (MPs), are tiny plastic fragments from 1 μm to 5 mm generally found in the aquatic environment which can be easily ingested by organisms and may cause chronic physical but also toxicological effects. Toxicological assays on fish cell lines are commonly used as an alternative tool to provide fast and reliable assessment of the toxic and ecotoxic properties of chemicals or mixtures. Rainbow trout liver cell line (RTLW-1) was used to evaluate the toxicity of pollutants sorbed to MPs sampled in sandy beaches from different islands around the world during the first Race for Water Odyssey in 2015. The collected MPs were analyzed for polymer composition and associated persistent organic pollutants: polycyclic aromatic hydrocarbons (PAHs), polychlorobiphenyls (PCBs) and dichlorodiphenyltrichloroethane (DDT). In addition, DMSO-extracts from virgin MPs, MPs artificially coated with B[a]P and environmental MPs were analyzed with different bioassays: MTT reduction assay (MTT), ethoxyresorufin-O-deethylase (EROD) assay and comet assay. Microplastics from sand beaches were dominated by polyethylene, followed by polypropylene fragments with variable proportions. Organic pollutants found on plastic from beach sampling was PAHs (2–71 ng g⁻¹). Samples from Bermuda (Somerset Long Bay) and Hawaii (Makapu'u) showed the highest concentration of PAHs and DDT respectively. No toxicity was observed for virgin microplastics. No cytotoxicity was observed on cells exposed to MP extract. However, EROD activity was induced and differently modulated depending on the MPs locations suggesting presence of different pollutants or additives in extract. DNA damage was observed after exposure to four microplastics samples on the six tested. Modification of EROD activity level and DNA damage rate highlight MPs extract toxicity on fish cell line.

Lipophilic marine algal toxins (LMATs) pose a potential threat to the health of marine shellfish consumers and marine breeding industries. In this study, LMATs in dissolved phases (DP) and particulate phases (PP) in the seawater of Jiaozhou Bay were accurately determined over four seasons to understand their composition, level, phase partitioning, spatiotemporal variation, and potential sources in aquatic environments of a typical semi-closed mariculture bay. Various LMATs, such as okadaic acid (OA), dinophysistoxin-1 (DTX1), dinophysistoxin-2 (DTX2), gymnodimine (GYM), 13-desmethyl spirolide C (SPX1), pectenotoxin-2 (PTX2), pectenotoxin-2 seco acid (PTX2 SA), and pectenotoxin-11 (PTX11), were detected in DP and PP; of these, OA and PTX2 were the dominant LMATs in DP and PP, respectively. The average proportion of ΣLMATs in DP (97.5%) was significantly higher than that in PP (2.5%), which indicates that LMATs are predominantly partitioned into DP. The total concentrations of LMATs in DP ranged from 4.16 ng/L to 23.19 ng/L (mean, 13.35 ng/L) over four seasons. The highest levels of LMATs in DP and PP were found in summer (mean, 16.71 ng/L) and spring, respectively, while the maximum variety of LMATs was found in autumn. This result suggests that seasonal changes could influence the composition, concentration, and phase partitioning of LMATs in aquatic environments of a coastal semi-closed mariculture bay. ΣLMAT concentrations were higher in the western region than in the eastern region of the bay, where shellfish may have a greater risk of exposure. Dinophysis acuminata, Dinophysis fortii, and Prorocentrum minimum were the potential sources of LMATs in the aquaculture seawater. Overall, various LMATs occurred in the semi-closed mariculture bay, and the persistence and bioavailability of these toxins in aquaculture seawater should be determined in future research.

Marine plastic debris, including microplastics (<5 mm in size), comprises a suite of chemical ingredients and sorbed chemical contaminants. Thus, microplastics are a potential, and debated, source of anthropogenic chemicals for bioaccumulation and biomagnification. Several studies have investigated the role of microplastics as a vector of contaminants to marine organisms via modeling exercises, laboratory experiments, and field studies. Here, we examined relationships among chemical contaminants and microplastics in lanternfish (family Myctophidae), an important link in marine food webs, from the North Pacific Ocean as a case study from the field. We compared the body burden of several chemical groups (bisphenol A [BPA], nonylphenol [4-NP], octylphenol [4n-OP], alkylphenol ethoxylates [APEs], pesticides, polychlorinated biphenyls [PCBs], and polybrominated diphenyl ethers [PBDEs]) in fish caught within and outside the North Pacific Subtropical Gyre where plastic is known to accumulate. We also tested whether there was a relationship between chemical concentrations in fish and plastic density at each sampling location. Mean concentrations of common plastic constituents (BPA, 4-NP, 4n-OP, APEs, and total PBDEs) were comparable between myctophids collected within and outside the North Pacific Gyre. Pesticides were higher in lanternfish caught outside the gyre and were associated with lower plastic density. Total PCBs were also higher in fish outside the gyre. In contrast, lower chlorinated PCB congeners were higher in fish residing in the accumulation zone and were correlated with higher plastic density. This finding is consistent with other studies demonstrating an association between lower chlorinated PCBs and plastics in biota and suggests that microplastic may be a transport mechanism for some chemicals in nature.

Silver nanoparticles (AgNPs) have adverse impacts on plants when released into environments, but their toxic mechanism is still a matter of debate. Here we present a combined analysis of physiology and transcriptome of Arabidopsis thaliana leaves exposure to 30 mg L−1 AgNPs and Ag+ for six days to explore the toxicity mechanism of AgNPs on Arabidopsis. Both transcriptomic and physiological results showed that AgNPs induced reactive oxygen species (ROS) accumulation and damaged photosynthesis. The toxicity of AgNPs is not merely attributable to Ag+ release and much higher photosynthetic toxicity and ROS accumulation were observed in 30 mg L−1 AgNPs than that in 0.12 mg L−1 Ag+. About 60% genes were similarly up- or down-regulated at the same concentration of AgNPs and Ag+ and these genes were enriched in photosynthesis and response to the stimulus. However, 302 genes, including those involved in glucosinolates synthesis, were specifically regulated under AgNPs treatments. In conclusion, more than the released Ag+, nanoparticle-specific effects are responsible for the toxicity of AgNPs in Arabidopsis thaliana.

Microplastic (MP) pollution is an emerging issue in aquatic sciences. Little comparative information currently exists about the problem in coastal systems exposed to different levels of human impact. Here we report a year-long study on the abundance of MP in the water column of three estuaries on the east-coast of Australia. The estuaries are subject to different scales of human impact; the Clyde estuary has little human modification, the Bega estuary has a small township and single wastewater treatment works discharging to its waters, and the Hunter estuary which has multiple townships, multiple wastewater treatment plants, and heavy industry. MP abundance followed an expected pattern with the lowest abundance in the low-impact Clyde estuary (98 part. m³), moderate levels of MP in the moderately impacted Bega estuary (246 part. m³), and high MP abundance in the highly impacted Hunter estuary (1032 part. m³). The majority of particles were <200 μm and fragment-like rather than fiber-like. MP abundance was positively related to maximum antecedent rainfall in the Bega estuary, however there are no clear environmental factors that could explain MP variation in the other systems. MP were generally higher in summer and following freshwater inflow events. On the Hunter estuary MP abundance was at times as high as zooplankton abundance, and within the range of numbers reported in other highly impacted systems globally. The results confirm that higher levels of human impact lead to greater plastic pollution and highlight the need to examine aquatic ecosystems under a range of conditions in order to adequately characterize the extent of MP pollution in rivers and coastal systems.

The ecological risk assessment (ERA) of polycyclic aromatic hydrocarbons (PAHs) is imperative due to their ubiquity and biological effects in aquatic organisms. We evaluated the seasonal levels and ERA of 16 priority PAHs in surface water, sediment and fish from four (4) anthropogenic-impacted zones of the Lagos lagoon, Nigeria. PAHs were analysed using GC-FID and standard guidelines were utilized for the ERA. Also, we developed a sediment to water-PAHs ratio and ranking of PAHs for the ERA. The dominant fish species were Sarotherodon melanotheron (Black-Jawed Tilapia), Gerres melanopterus (Gerres), Liza falcipinnis (Sicklefin Mullet) and Pseudotolithus elongatus (Bobo Croaker) at Ilaje, Iddo, Atlas cove and Apapa zones respectively. The range of sum PAHs was 195–1006 μg L⁻¹, 302–1290 μg kg⁻¹ and 8.80–26.1 μg kg⁻¹ in surface water, sediment and fish species respectively. Naphthalene was dominant in the surface water and sediment samples while 3-ring to 4-ring PAHs were predominant in fish species across the zones and seasons. The sediment to water-PAHs ratio was greater than 1 for sum PAHs and significantly higher (p < 0.05) in the wet season for specific PAHs across the zones and seasons. On the basis of the sediment PAHs level, Apapa zone was highly polluted with frequent biological effects while the other zones were moderately polluted with occasional biological effects across the zones and seasons. Fish species from all zones were minimally contaminated in both seasons except S. melanotheron which was not contaminated. The specific PAHs identified as ecological risk factors in the lagoon and ranking based on 50–75% recurrence in the ERA were; naphthalene, acenaphthene > acenaphthylene, fluorene, pyrene and benzo[a]anthracene. We recommend that the specific PAHs identified should form the basis for the establishment of environmental quality standards for individual PAHs in coastal waters based on the UN sustainable development goal 14 (life below water).