The effects of hypoxia on the larvae of two sub-tidal nassariid gastropods, Nassarius siquijorensis and N. conoidalis were compared so as to understand how the species-specific tolerance to hypoxia might have resulted in changes in the abundance and distribution of these two species in the hypoxic Tolo Harbour, Hong Kong, since the 1980s. Respiration rates of N. siquijorensis and N. conoidalis larvae were reduced at 4.5mg O₂ l⁻¹, or below, as compared with the normoxic control. Significant reduction in swimming velocity was also observed for 10-day old larvae which were exposed to <2.0mg O₂ l⁻¹ for N. siquijorensis and <1.0mg O₂ l⁻¹ for N. conoidalis. The 48h LC₅₀ values of N. siquijorensis and N. conoidalis larvae were 0.7 and 1.7mg O₂ l⁻¹, respectively. The results suggested that N. siquijorensis are more tolerant to hypoxia than N. conoidalis.

Linear Alkylbenzene Sulfonates (LAS) are ubiquitous surfactants. Traces can be found in coastal environments. Sorption and toxicity of C₁₂-LAS congeners were studied in controlled conditions (2–3500μg C₁₂LAS/L) in five marine phytoplanktonic species, using standardized methods. IC₅₀ values ranged from 0.5 to 2mg LAS/L. Sorption of ¹⁴C₁₂-6 LAS isomer was measured at environmentally relevant trace levels (4μg/L) using liquid scintillation counting. Steady-state sorption on algae was reached within 5h in the order dinoflagellate>diatoms>green algae. The sorption data, fitted a L-type Freundlich isotherm, indicating saturation. Desorption was rapid but a low LAS fraction was still sorbed after 24h. Toxic cell concentration was 0.38±0.09mg/g for the studied species. LAS toxicity results from sorption on biological membranes leading to non-specific disturbance of algal growth. Results indicate that LAS concentrations in coastal environments do not represent a risk for these organisms.

Located in southeastern Brazil, the Santos Estuary has the most important industrial and urban population area of South America. Since the 1950’s, increased urbanization and industrialization near the estuary margins has caused the degradation of mangroves and has increased the discharge of sewage and industrial effluents. The main objectives of this work were to determine the concentrations and sources of polycyclic aromatic hydrocarbons (PAHs) in sediment cores in order to investigate the input of these substances in the last 50years. The PAHs analyses indicated multiple sources of these compounds (oil and pyrolitic origin), basically anthropogenic contributions from biomass, coal and fossil fuels combustion. The distribution of PAHs in the cores was associated with the formation and development of Cubatão industrial complex and the Santos harbour, waste disposal, world oil crisis and the pollution control program, which results in the decrease of organic pollutants input in this area.

Hepatic lipidosis is a non-specific biomarker of effect from pollution exposure in fish. Fatty liver is often misdiagnosed or overlooked in histological assessments due to the decreasing application of specific fat procedures and stains. For example, ethanol dehydration in standard paraffin processing removes lipids, leaving vacuoles of which the precise nature is unknown. Lipids can be identified using osmium post-fixation in semi-thin resin sections or transmission electron microscopy. However, both are expensive and technically demanding procedures, often not available for routine environmental risk assessment and monitoring programs. The current emphasis to reduce and refine animal toxicity testing, requires refinement of the suite of histopathological techniques currently available to maximize information gained from using fish for toxicity testing and as bio-indicators of environmental quality. This investigation has successfully modified an osmium post-fixation technique to conserve lipids in paraffin-embedded tissues using medaka (Oryzias latipes) eleutheroembryos and eggs (embryos) as lipid rich models.

Nineteen trace elements were determined in liver, muscle, kidney, gonads, and hair of 18 harp seals (Phoca groenlandica) from Pangnirtung in the Baffin Island, Canada. Concentrations of V, Mn, Fe, Cu, Mo, Ag, and Hg in the liver, Co, Cd, and Tl in the kidney, and Ba and Pb in the hair were significantly higher than those in other tissues. Significant positive correlations between Hg concentrations in the hair, and liver, kidney and testis imply usefulness of the hair sample for non-destructive monitoring of Hg in the harp seals. It is suggested that whereas Hg preferentially accumulates in the liver, the accumulation in other tissues is induced at higher hepatic Hg levels. In contrast, Se may not be accumulated in other tissues compared with the liver even at higher hepatic Hg levels because of the presence of excess Se for Hg detoxification in other tissues.

Typhoon Morakot pounded Taiwan in 2009 with record-breaking rainfall, washing an unprecedented amount of driftwood into the sea that was partially deposited at the coastal areas. According to the satellite imagery analysis, more than three million trees fell and were washed away to occupy 83.2% of the Taiwanese coastline, including 52 fishing harbors. The amount cleaned-up was only 1/7 of the total coastal driftwood. It was found that the amount of coastal driftwood is not only related to the amount of precipitation but is also related to the distance from the location of the landslide to the river mouth and to the landslide area. The amount of accumulated coastal driftwood demonstrated log-profile declines with increasing distance to the river mouth. Nearshore current and wave motion are the critical factors for driftwood deposition. Much of the driftwood washed into the sea harmed the tourism and fishing industries, endangered navigation and oceanic activities, and impacted the marine environment and ecosystem.

Endocrine disruption, in particular disruption by estrogen-active compounds, has been identified as an important ecotoxicological hazard in the aquatic environment. Research on the impact of endocrine disrupting compounds (EDCs) on wildlife has focused on disturbances of the reproductive system. However, there is increasing evidence that EDCs affect a variety of physiological systems other than the reproductive system. Here, we discuss if EDCs may be able to affect the immune system of fish, as this would have direct implications for individual fitness and population growth. Evidence suggesting an immunomodulatory role of estrogens in fish comes from the following findings: (a) estrogen receptors are expressed in piscine immune organs, (b) immune gene expression is modulated by estrogen exposure, and (c) pathogen susceptibility of fish increases under estrogen exposure.

Ballast water exchange was measured for the first time in Bilbao Harbour, one of the most active of northern Spain. Between 1997 and 2006, 41,900,980.34 ballast water tn were loaded and 13,272,709 tn were discharged. Bilbao Harbour appears to be mainly a source of ballast water, 90% of which would be discharged in European harbours. We estimated that vessels carrying liquid and solid bulk have higher probabilities of exporting ballast water, whereas those with liquid bulk and containers are more likely to introduce it. From 30 potentially harmful phytoplankton species identified to date near harbour facilities, there would be a high risk of exporting at least Alexandrium minutum, Dinophysis sp., Heterosigma akashiwo, Karlodinium sp., Ostreopsis cf. siamensis, Pfiesteria-like and Prorocentrum minimum. Invasion risk by ballast water was tested by analyzing the response of six strains of H. akashiwo from different geographic areas to varying salinity. Results show that successful growth of foreign strains would be possible.

Proteomics is a new and promising approach to evaluate potential effects of pollution. In order to investigate if there is a direct link between the protein expression profiles obtained by the SELDI–TOF MS technology and effects observed at the organism level in fish, plasma samples from unexposed and 20ppb alkylphenol exposed female Atlantic cod (Gadus morhua) with high phenotypic variation in gonadosomatic index (GSI) were analyzed by SELDI–TOF MS. Principle component analysis (PCA) showed that the major proteomic variation present in the dataset (i.e. 23.6%) could be significantly correlated to the individual variation in GSI, which indicates that SELDI–TOF MS data can reflect effects observed at higher levels of organization in fish. Further exploration of the other principal components revealed an additional proteomic pattern specific for the alkylphenol exposed females. Hence, this study supports the usefulness of SELDI–TOF MS as a proteomic tool in ecotoxicological research.

PCBs equivalent to Aroclor 1242 and 1254 in soft tissues of oysters Crassostrea rivularis from the Guangdong coast, China, were measured using a GC-ECD. PCB concentrations (ngg⁻¹d.w.) ranged from 30 to 2040 with an average of 315 in 2003–2007, and 0.35–1.43 with an average of 0.56 in 1989–1991. Annual averages were 0.61, 0.65, 313, 290 and 342ngg⁻¹, respectively in 1989, 1991, 2003, 2006 and 2007. In east Guangdong, the Pearl River Estuary, and west Guangdong, regional means were 444, 273, 194ngg⁻¹, respectively in 2003–2007, and 0.43, 0.78, 0.38ngg⁻¹, respectively in 1989–1991, indicating PCB levels in oysters have risen greatly by ratios of 350–1032 compared with earlier data. PCB sources may derive from older transformers, printing materials and pressworks. Fortunately, current residual PCBs in the oysters were 0.004–0.253mgkg⁻¹ (w.w.), still below hygienic criteria of China and developed countries.