In soils, enzymes are crucial to catalyzing reactions and cycling elements such as carbon (C), nitrogen (N), and phosphorus (P). Although these soil enzymes are sensitive to metals, they are often disregarded in risk assessments, and regulatory laws governing their existence are unclear. Nevertheless, there is a need to develop regulatory standards for metal mixtures that protect biogeochemical cycles because soil serve as a sink for metals and exposures occur as mixtures. Using a fixed ratio ray design, we investigated the effects of 5 single metals and 10 quinary mixtures of Zn, Cu, Ni, Pb, and Co metal oxides on two soil enzymes (i.e., acid phosphatases [ACP] and beta glucosidases [BGD]) in two acidic Canadian soils (S1: acid sandy forest soil, and S2: acid sandy arable soil), closely matched to EU REACH standard soils. Compared to BGD, ACP was generally the more sensitive enzyme to both the single metals and the metal mixtures. The effective concentration inhibiting 50% enzyme activity (EC₅₀) estimates for single Cu (2.1–160.7 mmol kg⁻¹) and Ni (12–272 mmol kg⁻¹) showed that those were the most toxic to both enzymes in both soils. For metal mixtures, response addition (RA) was more conservative in predicting metal effects compared to concentration addition (CA). For both additivity models, antagonism was observed except at lower concentrations (≤10,000 mg/kg) where synergism was observed. At higher concentrations (>10,000 mg/kg), free and CaCl₂ extractable Cu protected both enzymes against the toxicity of other metals in the mixture. The results suggest that assuming CA at concentrations less than EC₅₀ does not protect biogeochemical cycling of C and P. And Cu in soil may protect soil enzymes from other toxic metals and thus may have an overall positive role.

Many organic materials have been used to decrease heavy-metal bioavailability in soil via in-situ remediation due to its high efficiency and easy operation; meanwhile, cheap materials have also been pursued to decrease the cost of remediation. Agricultural wastes exhibit their potential in remediation materials due to their low cost; however, raw agricultural wastes have a low ability to immobilize heavy metals in soil. Attempts have been made to modify agricultural wastes to improve the efficiency of heavy-metal passivation. In this study, novel agricultural waste-based materials, raw sugarcane bagasse (SB), citric acid modified (SSB) and citric-acid/Fe₃O₄ modified (MSB) sugarcane bagasse at 0.5% and 1% addition rates, were compared for their effectiveness in soil Cd passivation and Cd accumulations in pakchoi plants in a 30-day pot experiment. The addition of SB did not decrease soil bioavailable Cd effectively and slightly decreased Cd accumulation in plant roots and leaves. In comparison, SSB and MSB exhibited a great potential to decrease the transformation, translocation and accumulation of Cd with the decrease being greater at 1% than 0.5% rate in the soil-pakchoi system. For example, the addition of SSB and MSB at 0.5% decreased the concentration of Cd in leaves by 10%, and 16%, and at 1% decreased the concentration by 25% and 30%, respectively. High pH and abundant functional groups of three amendments played important roles in Cd immobilization. The enhanced microbial activities might also contribute to Cd passivation. However, plant growth was decreased in the amended treatments except SSB at 0.5% rate. The results suggest that citric-acid-modified sugarcane bagasse at addition rate of 0.5% has a potential to immobilize Cd in soil and decrease Cd accumulation in edible part of pakchoi effectively without decreasing vegetable growth.

The current study investigated seasonal fluctuations in diversity of fish and heavy metal concentrations in coastal areas, as well as the possible human health risks associated by the heavy metals (Mercury, Lead, Chromium, Cadmium, Copper and Zinc). From five different locations across the coastal area, 44 finfish species from 11 orders and 33 families were collected. Four finfish species such as Mugil cephalus, Lates calcarifer, Etroplus suratensis, and Chanos chanos were used to estimate and assess the heavy metal concentrations based on abundance and distribution across coastal area. Results revealed that the metal concentration in these fish species, water, and sediment were all found to be significantly comparable. During the southwest monsoon season, the highest concentrations of metals were found in Chanos chanos, Mugil cephalus, and Lates calcarifer. A hazard index and a target hazard quotient were calculated to determine the human-related health risk. Except for Hg and Cd in children, the anthropological health hazard assessment revealed that most element exposure doses are safe for both children and adults.

The stable stabilization of uranium (U) in iron (Fe) containing environments is restricted by the reoxidation of UO₂. In the current study, based on air reoxidation tests, we propose a novel two steps accumulation method to enrich microbial consortia from paddy soil. The constructed microbial consortia, denoted as the Fe–U bacteria, can co-precipitate U and Fe to form stable Fe–U solids. Column experiments running for 4 months demonstrated the production of U(IV)–O–Fe(II) precipitates containing maximum of 39.51% uranium in the presence of Fe–U bacteria. The reoxidation experiments revealed the U(IV)–O–Fe(II) precipitates were more stable than UO₂. 16S rDNA high throughput sequencing analysis demonstrated that Acinetobacter and Stenotrophomonas were responsible for Fe and U precipitation, while, Caulobacteraceae and Aminobacter were crucial for the formation of U(VI)-PO₄ chemicals. The proposed two steps accumulation method has an extraordinary application potential in stable immobilization of uranium in iron containing environments.

Microorganisms are essential for modifying arsenic morphology, mobility, and toxicity. Still, knowledge of the microorganisms responsible for arsenic metabolism in specific arsenic-contaminated fields, such as metallurgical plants is limited. We sampled on-field soils from three depths at 70 day intervals to explore the distribution and transformation of arsenic in the soil. Arsenic-metabolizing microorganisms were identified from the mapped gene sequences. Arsenic metabolism pathways were constructed with metagenomics and AsChip analysis (a high-throughput qPCR chip for arsenic metabolism genes). It has been shown in the result that 350 genera of arsenic-metabolizing microorganisms carrying 17 arsenic metabolism genes in field soils were identified, as relevant to arsenic reduction, arsenic methylation, arsenic respiration, and arsenic oxidation, respectively. Arsenic reduction genes were the only genes shared by the 10 high-ranking arsenic-metabolizing microorganisms. Arsenic reduction genes (arsABCDRT and acr3) accounted for 73.47%–78.11% of all arsenic metabolism genes. Such genes dominated arsenic metabolism, mediating the reduction of 14.11%–19.86% of As(V) to As(III) in 0–100 cm soils. Arsenic reduction disrupts microbial energy metabolism, DNA replication and repair and membrane transport. Arsenic reduction led to a significant decrease in the abundance of 17 arsenic metabolism genes (p < 0.0001). The critical role of arsenic-reducing microorganisms in the migration and transformation of arsenic in metallurgical field soils, was emphasized with such results. These results were of pronounced significance for understanding the transformation behavior of arsenic and the precise regulation of arsenic in field soil.

Heavy metals and organic dyes are the major source of water pollution. Herein, a trifunctional β−cyclodextrin−ethylenediaminetetraacetic acid−chitosan (β−CD−EDTA−CS) polymer was synthesized using an easy and simple chemical route by the reaction of activated β−CD with CS through EDTA as a cross-linker (amidation reaction) for the removal of inorganic and organic pollutants from aqueous solution under different parameters such as pH, time effect, initial concentration, reusability, etc. The synthesized adsorbent was characterized using powder X-ray diffraction, Fourier transform infrared spectroscopy, field scanning electron microscopy, energy dispersive spectroscopy, Brunauer-Emmett-Teller (BET), thermogravimetric analyzer techniques to investigate their structural, functional, morphological, elemental compositions, surface area and thermal properties, respectively. Two types of heavy metals, i.e., mercury (Hg²⁺) and cadmium (Cd²⁺), and three organic dyes, i.e., methylene blue (MB), crystal violet (CV) and safranin O (SO) were chosen as inorganic and organic pollutants, respectively, to study the adsorption capacity of β-CD-EDTA-CS in aqueous solution. The β-CD-EDTA-CS shows monolayer adsorption capacity 346.30 ± 14.0 and 202.90 ± 13.90 mg g⁻¹ for Hg²⁺ and Cd²⁺, respectively, and a heterogeneous adsorption capacity 107.20 ± 5.70, 77.40 ± 5.30 and 55.30 ± 3.60 mg g⁻¹ for MB, CV and SO, respectively. Kinetics results followed pseudo-second order (PSO) kinetics behavior for both metal ions and dyes, and higher rate constants values (0.00161–0.00368 g mg⁻¹ min⁻¹) for dyes confirmed the cavitation of organic dyes (physisorption). In addition, we have also demonstrated the performance of β-CD-EDTA-CS for the of four heavy metals Hg²⁺, Cd²⁺, Ni²⁺, and Cu²⁺ and three dyes MB, CV, and SO in secondary treated wastewater. Findings of this study indicate that β-CD-EDTA-CS simple and essay to synthesize and can be use in wastewater treatment.

Oyster farming for black pearl production is central in French Polynesia. It is the second source of national income and provides substantial job opportunities, notably in remote atolls. However, this sector has been undermined by successive crises, such as mass-mortalities of wild and farmed oyster stocks that have impacted entire lagoons. An option to revive the activity consists of reintroducing oysters in strategic benthic locations selected to maximize reproduction and dispersal of larvae throughout the lagoon, hence promoting recolonization and spat collection for farming. For Takaroa, a Tuamotu atoll recently impacted by mortalities, a systematic prioritization approach identified these restocking sites, using environmental and socio-economic criteria such as: location of suitable habitats for oyster settlement, larval connectivity estimated from hydrodynamic circulation model, farming waste accumulation, and opportunity cost to fishers and farmers who lose access to restocking areas. This approach provides managers with a portfolio of restocking options.

Plant culture integration within aquaculture activities is a topic of recent interest with economic and environmental benefits. Shrimp farming activities generate nutrient-rich waste trapped in the sediments of farming ponds or release in the mangrove area. Thus, we investigate if the halophytes species naturally growing around the pond can use nitrogen and carbon from shrimp farming for remediation purposes. Halophyte biomasses and sediments influenced by shrimp farm effluents, were collected in two farms in New-Caledonia. All samples were analyzed for their C and N stable isotopic composition and N content. Higher δ15N values were found in plants influenced by shrimp farm water thus evidenced their abilities to take nutrient derived from shrimp farming. Deep root species Chenopodium murale, Atriplex jubata, Suaeda australis and Enchylaena tomentosa appears more efficient for shrimp pond remediation. This work demonstrates that halophytes cultivation in shrimp ponds with sediments, could be effective for the pond's remediation.

This editorial presents results of the MANA (MANagement of Atolls) project compiled in the form of a Marine Pollution Bulletin collection of 14 articles. MANA is a project funded by the French Agence National pour la Recherche that specifically addresses the development of knowledge and management tools for pearl farming atolls, with a focus on the spat collecting activity in French Polynesia. The 14 papers cover the range of thematic tasks described in the initial project, including atoll geomorphology and bathymetry, climate forcing, atoll lagoon and rim hydrodynamics, typology of atolls, evaluation of remote sensing data for monitoring atoll lagoons, and development of numerical models and spatially-explicit tools that altogether have contributed to the applied objectives. In addition, this editorial draws an update on the pearl farming industry in French Polynesia with the latest statistics, and discusses the next targeted priorities for research programs focusing on pearl farming atolls.

French Polynesia atolls are spread on a vast 2300 by 1200 km Central Pacific Ocean area exposed to spatially and temporally dependent wave forcing. They also have a wide range of closed to open morphologies and several have been suitable to develop from black-lipped pearl oysters a substantial pearl farming activity in the past 30 years, representing nowadays the 2nd source of income for French Polynesia. Considering here only the component of lagoon renewal that is driven by waves, we investigate for 74 atolls different lagoon renewal metrics using 20 years of wave model data at 0.05° spatial resolution. Wavelet spectral analyses highlight that atolls, even in close vicinity, can be exposed to different and characteristic periodicities in wave-driven flows and water renewal. These characteristics are discussed in relation to pearl farming atolls, including atolls known to be efficient oyster spat producers, a critical activity for pearl farming sustainability.