Sedum alfredii is a Cd/Zn hyperaccumulator native to China, which was collected from a mined area where Mn content in soil was extremely high, together with Zn and Cd content. We investigated the tolerance and accumulation ability of Mn and its possible association with Cd hyperaccumulation in this plant species by using MP-AES, SR-μ-XRF, and RT-PCR. The results showed that the hyperaccumulating ecotype (HE) S. alfredii exhibited high tolerance to Mn and accumulating around 10,000 and 12,000 mg kg⁻¹ Mn in roots and shoots, respectively, without exhibiting toxicity under 5000 mg kg⁻¹ Mn treatment for 4 weeks. Exposure to Cd significantly reduced plant uptake of Mn. In contrast, exogenous Mn application significantly improved root uptake and root-to-shoot translocation of Cd, resulting in the increased Cd accumulation in the shoots of HE S. alfredii. SR-μ-XRF analysis demonstrated that high Mn (20 μM) exposure resulted in higher intensities of Cd localized in both stem vascular bundles and cortex, as well as leaf mesophyll cells, than in those treated with low Mn levels (0.2 μM or 2.0 μM). RT-PCR analysis of several genes possibly involved in Mn/Cd transportation showed that expression of SaNramp3 in roots was significantly reduced under high Mn exposure. These results suggested a significant interaction between Cd and Mn in the HE S. alfredii plants, possibly through their competition for transporters and theoretically provided a strategy to improve the efficiency of Cd extraction from polluted soils by this plant species, after using appropriate nutrient management of Mn.

Hybrid electric vehicle (HEV) technology is critical to reduce the impact of the internal combustion engines on air pollution and greenhouse gases. HEVs have an advantage in market penetration due to their lower cost and higher driving range compared to battery electric vehicles (BEVs). On the other hand, HEVs use an internal combustion engine and still emit air pollutants. It is hypothesized that HEV performance is impacted by the weather conditions as a result of many factors. It was beyond the scope of this work to systematically evaluate all factors so instead we measured emissions from two vehicles driving city and highway routes in Minneapolis, Minnesota in the winter (−5 °C) and looked for major differences in emissions relative to each vehicle and relative to results that would be obtained from a chassis dynamometer in a controlled laboratory setting at a higher temperature approximately 20 °C). The study then looked to associate differences in emissions with the prevailing conditions to gain new insights. Emissions of interest included the total particle number (TPN), solid particle number (SPN), particulate matter mass (PM), and NOx. One key difference in vehicle engine technology was PFI (port fuel injection) versus GDI (gasoline direct injection). We found the frequency at which the Prius hybrid engine reignited was much higher than the Sonata for city and highway driving, although for both vehicles the catalyst temperature remained high and appeared to be unaffected by the reignitions, despite the cold weather. For most conditions, the Prius emitted more NOₓ but fewer particles than the Sonata. In some cases, NOₓ and particle emissions exceeded the most comparable laboratory-based emissions standards.

Biomass burning tracers have been widely used to identify biomass burning types, but such tools can sometimes cause large uncertainties in the source attribution studies of PM₂.₅ (particles with an aerodynamic diameter of smaller than 2.5 μm). To quantify the relative importance of the major biomass burning tracers in PM₂.₅ released from biofuels combusted in the North China Plain, combustion experiments under the smoldering and flaming combustion conditions were conducted using nine types of typical household biofuels including two types of agricultural wastes, five types of hardwoods, one softwood, and one mixed wood briquette. PM₂.₅ samples were collected from the combustion experiments and source profiles of PM₂.₅ were thus determined for various biofuels under the two different combustion conditions. Carbonaceous species including organic carbon (OC) and elemental carbon (EC) were the major chemical components of the PM₂.₅ released from combustion of all the tested biofuels, with mass fractions of 37–45% and 4–7% under the smoldering condition and 11–25% and 7–29% under the flaming condition, respectively. Higher mass fractions of water-soluble inorganic ions (WSIIs, e.g., K⁺ and Cl⁻) in PM₂.₅ were observed under the flaming than smoldering combustion condition, while anhydrosugars (levoglucosan (LG) and mannosan (MN)) presented in an opposite pattern. The average LG/MN ratio in PM₂.₅ changed significantly with biofuel type (20–55 for agricultural wastes, 10–22 for hardwoods (except elm) and 3–6 for softwood), but varied little with combustion condition. In contrast, the K⁺/LG ratio in PM₂.₅ varied significantly between smoldering (<0.2) and flaming (>0.6) combustion conditions for all the biofuel types except softwood. Results from this study suggested that the ratio LG/MN was the best tracer for identifying the biofuel types and the ratio K⁺/LG is suitable for identifying the combustion conditions in this region.

Previous studies assessing excessive proliferation of phytoplankton (EPP) in lakes are generally based on single investigation and focused on limited environmental factors; meanwhile, less attention has been paid to lakes susceptibility to EPP. Here, we identify the priority of lakes for EPP control in a basin by assessing EPP in multiple lakes and identify the key factors related to lakes’ vulnerability to EPP. Field measurements, as well as multi-source survey data acquisition were conducted for 63 shallow lakes in the middle-lower Yangtze River basin. Resource-use efficiency by phytoplankton (RUE) was then used to represent lake susceptibility to EPP. Generalized linear models were used to assess the relative importance of environmental factors for RUE. We found that most lakes (76.19 %) were not suitable for recreation, due to health concern attributed to irritative or allergenic risk caused by EPP. Phosphorus was the primary limiting nutrient for EPP (74.60 % of lakes) which should be limited to < 0.09 mg/L. The linear model that included latitude, particulate matter 10, and precipitation explained 27.60 % of the variation of RUETP among lakes. In contrast, the linear model that included ozone, Secchi depth, and wind speed explained 19.41 % of the variation of RUETN among lakes. The key factor related to RUETP and RUETN was particulate matter 10 and ozone, respectively, both of which potentially increase RUE or reflect it. Our results suggest that integrating multiple survey datasets is critical for lakes EPP assessment in a basin, while lakes impacted by air pollution are a high priority for EPP control.

Nitrogen (N) addition and mowing can significantly influence micronutrient cycling in grassland ecosystems. It remains largely unknown about how different forms of added N affect micronutrient status in plant-soil systems. We examined the effects of different N compounds of (NH₄)₂SO₄, NH₄NO₃, and urea with and without mowing on micronutrient Fe, Mn, Cu, and Zn in soil-plant systems in a meadow steppe. The results showed that (NH₄)₂SO₄ addition had a stronger negative effect on soil pH compared with NH₄NO₃ and urea, resulting in higher increases in soil available Fe and Mn herein. Nitrogen addition decreased plant community-level biomass weighted (hereafter referred to as community-level) Fe concentration but increased Mn concentration, with a greater effect under (NH₄)₂SO₄ addition. Community-level Cu concentration increased with (NH₄)₂SO₄ and NH₄NO₃ addition only under mowing treatment. Mowing synergistically interacted with urea addition to increase community-level Mn and Zn concentrations even with decreased soil organic matter, possibly because of compensatory plant growth and thus higher plant nutrient uptake intensity under mowing treatment. Overall, responses of plant-soil micronutrients to N addition varied with mowing and different N compounds, which were mainly regulated by soil physicochemical properties and plant growth. Different magnitude of micronutrient responses in plants and soils shed light on the necessity to consider the role of various N compounds in biogeochemical models when projecting the effects of N enrichment on grassland ecosystems.

Few studies have investigated the effect of personal PM₂.₅ and PM₁ exposures on heart rate variability (HRV) for a community-based population, especially in Asia. This study evaluates the effects of personal PM₂.₅ and PM₁ exposure on HRV during two seasons for 35 healthy adults living in an urban community in Taiwan. The low-cost sensing (LCS) devices were used to monitor the PM levels and HRV, respectively, for two consecutive days. The mean PM₂.₅ and PM₁ concentrations were 13.7 ± 11.4 and 12.7 ± 10.5 μg/m³ (mean ± standard deviation), respectively. Incense burning was the source that contributed most to the PM₂.₅ and PM₁ concentrations, around 9.2 μg/m³, while environmental tobacco smoke exposure had the greatest impacts on HRV indices, being associated with the highest decrease of 20.2% for high-frequency power (HF). The results indicate that an increase in PM₂.₅ concentrations of one interquartile range (8.7 μg/m³) was associated with a change of −1.92% in HF and 1.60% in ratio of LF to HF power (LF/HF). Impacts on HRV for PM₁ were similar to those for PM₂.₅. An increase in PM₁ concentrations of one interquartile range (8.7 μg/m³) was associated with a change of −0.645% in SDNN, −1.82% in HF and 1.54% in LF/HF. Stronger immediate and lag effects of PM₂.₅ exposure on HRV were observed in overweight/obese subjects (body mass index (BMI) ≥24 kg/m²) compared to the normal-weight group (BMI <24 kg/m²). These results indicate that even low-level PM concentrations can still cause changes in HRV, especially for the overweight/obese population.

Gabapentin-lactam (GBP-L) is a transformation product (TP) of gabapentin (GBP), a widely used anti-epileptic pharmaceutical. Due to its high persistence, GBP-L has been frequently detected in the surface water. However, the effects of GBP-L on aquatic organisms have not been thoroughly investigated. In the present study, zebrafish (Danio rerio) embryos as a model organism were used to study the impacts of GBP-L in terms of embryos LC₅₀, spontaneous movement at 24 hpf (hours post fertilization), heartbeat rates at 48 hpf, and body length at 72 hpf, with the concentrations of GBP-L down to 0.01 μg/L, covering its environmental concentrations. Various biomarkers from nervous, antioxidant and immune systems of zebrafish larvae were analyzed, including acetylcholinesterase, acetylcholine, dopamine, gamma-aminobutyric acid, superoxide dismutase, catalase, glutathione S-transferase, C reactive protein, and lysozyme, to assess its toxicity on these systems. RT-qPCR was then used to further verify the results and explain the toxicological mechanism at the gene level. The results demonstrated that GBP-L is much more toxic than its parent compound, and could lead to adverse impacts on the aquatic organisms even at every low concentrations.

Sessile benthic organisms are considered good bioindicators for monitoring environmental quality of coastal ecosystems. However, these environments are impacted by new pollutants such as microplastics (MPs), where there is limited information about organisms that can be used as reliable bioindicators of these emerging contaminants. We evaluated MP concentrations in three compartments: surface sediment, water and in three marine sponge species (Haliclona implexiformis, Halichondria melanadocia and Amorphinopsis atlantica), to determine whether these organisms accumulate MPs and reflect their possible sources. Results showed MPs in all three compartments. Average concentrations ranged from 1861 to 3456 items kg⁻¹ of dry weight in marine sponges, 130 to 287 items L⁻¹ in water and 6 to 11 items kg⁻¹ in sediment. The maximum MP concentration was in the sponge A. atlantica, which registered 5000 items kg⁻¹ of dry weight, in water was 670 items L⁻¹ and in sediment was 28 items kg⁻¹, these values were found in the disturbed study area. The three sponge species exhibited MP bioaccumulation and showed significant differences between disturbed and pristine sites (F = 11.2, p < 0.05), suggesting their use as bioindicators of MP.

This work demonstrated the development of nanofiber templated metal oxide nanocomposites by hydrothermal and calcination methods for photocatalytic degradation using Congo red (CR) as model pollutant. Herein, we developed PAN/CuO–ZnO nanocomposites by the electrospinning technique followed by heat treatment process i.e hydrothermal and calcination. The obtained nanofibrous composites were characterized by various analytical techniques such as X-Ray Diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), Thermogravimetric analysis (TG), High-resolution transmission electron microscopy (HRTEM), X-ray photoelectron spectroscopy (XPS), Photoluminescence (PL) and UV–Vis diffuse reflectance spectroscopy (DRS) studies. The results demostrated that the nanocomposites obtained through calcination possess better optical response with robust electronic structures. This is due to the better charge separation of excited electron-hole pairs of p-n heterostructured PAN/CuO–ZnO hybrid nanocomposites. The photocatalytic efficiency is found to be 98% and 93% for nanocomposites obtained through calcination and hydrothermal methods respectively. The reusability studies confirmed the stability and viability of multiple utilizations of photocatalysts. Furthermore, the photocatalytic mechanism corroborated the photocatalytic properties of the integrated facile nanofibrous-metallic (PAN/CuO–ZnO) composites and hence can be implemented in water remediation effectively.