Recent studies suggests that air pollution, from among others road traffic, can influence growth and development of the human foetus during pregnancy. The effects of air pollution from heavy industry on birth outcomes have been investigated scarcely.Our aim was to investigate the associations of air pollution from heavy industry on birth outcomes.A cross-sectional study was conducted among 4488 singleton live births (2012–2017) in the vicinity of a large industrial area in the Netherlands. Information from the birth registration was linked with a dispersion model to characterize annual individual-level exposure of pregnant mothers to air pollutants from industry in the area. Associations between particulate matter (PM₁₀), nitrogen oxides (NOX), sulphur dioxide (SO₂), and volatile organic compounds (VOC) with low birth weight (LBW), preterm birth (PTB), and small for gestational age (SGA) were investigated by logistic regression analysis and with gestational age, birth weight, birth length, and head circumference by linear regression analysis.Exposures to NOX, SO₂, and VOC (per interquartile range of 1.16, 0.42, and 0.97 μg/m³ respectively) during pregnancy were associated with LBW (OR 1.20, 95%CI 1.06–1.35, OR 1.20, 95%CI 1.00–1.43, and OR 1.21, 95%CI 1.08–1.35 respectively). NOX and VOC were also associated with PTB (OR 1.14, 95%CI 1.01–1.29 and OR 1.17, 95%CI 1.04–1.31 respectively). Associations between exposure to air pollution and birth weight, birth length, and head circumference were statistically significant. Higher exposure to PM₁₀, NOX, SO₂ and VOC (per interquartile range of 0.41, 1.16, 0.42, and 0.97 μg/m³ respectively) was associated with reduced birth weight of 21 g to 30 g.The 90th percentile industry-related PM₁₀ exposure corresponded with an average birth weight decrease of 74 g.

Environmental exposure to pollution generated by mining and burning coal is inevitable for people living nearby. Therefore, the aim of this study was to evaluate the influence of coal dust on health conditions and genomic instability of individuals who live near coal mines and thermoelectric power plants, and to relate the results to inorganic elements and inflammatory responses. Thus, we evaluated 284 individuals from four cities in the south of Brazil around a region with coal mines and a thermoelectric power plant (one city was considered a negative control). The results of the Comet assay and Micronucleus (MN) test did not show a genotoxic or mutagenic effect related to environmental exposure to coal, but the inflammatory cytokine tumor necrosis factor-α (TNF-α) was increased in all cities around the power plant when compared to the control conditions. Higher levels of MN were associated with body mass index and cardiovascular risk, and higher levels of Damage Index (DI), TNF-α and interleukin1β (IL-1β) with number of cigarettes/day. Principal component analysis (PCA) was used to integrate DNA damage and inflammatory results with inorganic elements. This study also demonstrated the relationship between zinc and MN, copper, and interleukin10 (IL-10), and among silicon and sulfur with DI and nucleoplasmic bridge. A relationship was also observed between the reduction of inorganic elements and both aging and quality of health. The use of different methodologies and the relationship between the results obtained in these studies, including different lifestyles, can increase the understanding of the interaction between this mineral and the health status of residents of regions affected by coal pollution.

Frequent and intense storm disturbances can have widespread and strong effects on the nitrogen and iron cycles and their associated microbial communities in estuary systems. A three-year investigation was conducted in the Pearl River and Zhanjiang estuaries in Guangdong Province, China through repeated sampling at three timepoints, defined as pre-storm (<1 month before storm), post-storm (<1 month after storm), and non-storm (6–8 months after storm). Increased nutrient concentrations (total organic carbon, nitrate, nitrite, ammonium, and sulfate) in both the sediment and water column were observed immediately after storm. The microbial community experienced extensive and immediate changes determined by an observed composition shift in the nitrogen and iron-cycling microbiomes. Analysis of sediment samples displayed a shift from nitrogen-to sulfur-cycling microorganisms and an increase in microbial interactions that were not observed in the water column. The chemical profile and microbial community components both returned to baseline conditions 6–8 months following storm disturbance. Finally, significant correlations were found between chemical and microbial data, suggesting that niche-sharing microbes may respond similarly to stimuli that impact their ecosystem. Increases in nutrient availability can favor the abundance of specific taxa, as demonstrated by an increase in Acidimicrobium that affect both nitrogen and iron cycling.

A native strain of Bacillus paramycoides isolated from the leachate of coal mine overburden rocks was investigated for its potential to produce selenium nanoparticles (SeNPs) by biogenic reduction of selenite, one of the most toxic forms of selenium. 16S rDNA sequencing was used to identify the bacterial strain (SP3). The SeNPs were characterized using spectroscopic (UV–Vis absorbance, dynamic light scattering, X-ray diffraction, and Raman), surface charge measurement (zeta potential), and ultramicroscopic (FESEM, EDX, FETEM) analyses. SP3 exhibited extremely high selenite tolerance (1000 mM) and reduced 10 mM selenite under 72 h to produce spherical monodisperse SeNPs with an average size of 149.1 ± 29 nm. FTIR analyses indicated exopolysaccharides coating the surface of SeNPs, which imparted a charge of −29.9 mV (zeta potential). The XRD and Raman spectra revealed the SeNPs to be amorphous. Furthermore, biochemical assays and microscopic studies suggest that selenite was reduced by membrane reductases. This study reports, for the first time, the reduction of selenite and biosynthesis of SeNPs by B. paramycoides, a recently discovered bacterium. The results suggest that B. paramycoides SP3 could be exploited for eco-friendly removal of selenite from contaminated sites with the concomitant biosynthesis of SeNPs.

Guadipyr, which combines neonicotinoid and semicarbazone functional groups in one molecule, exhibits good activity on several pests and high acute and chronic toxicity to silkworms (Bombyx mori). In this report, the effects of low-dose guadipyr on the midgut microbiota and immune system of silkworms were studied. Results showed that the structure and richness of the midgut microbiota of silkworms were altered after being treated with 5.25 mg/L (1/10 of LC₅₀) of guadipyr. The abundance of Pseudomonas was evidently increased, whereas Curvibacter was substantially reduced, which might be related to the growth and immunity of silkworms. The expression of key genes in the Toll, IMD, and JAK/STAT pathways, which ultimately led to the downregulation of antimicrobial peptide genes (AMPs), such as CecA, Defensin1, Leb, and glv2, was reduced upon guadipyr exposure. Simultaneously, the suppression of steroid hormone 20-hydroxyecdysone receptor and response genes, such as BR-C Z4, was detected in the exposed groups. The decreased expression of these immune regulatory pathway-related and 20-hydroxyecdysone signal pathway-related genes indicated that the immune system of silkworms was affected by low-dose guadipyr. Our results revealed the negative effects of guadipyr on silkworms and highlighted the unneglectable toxicity of low-dose guadipyr to this economic insect. Given the risk, it is necessary to control the application of guadipyr in or around the mulberry fields.

With the implementation of clean air strategies, PM₂.₅ pollution abatement has been observed in the “2 + 26” cities in the Beijing-Tianjin-Hebei (BTH) region (referred to as the BTH2+26) and their surrounding areas. To identify the drivers for PM₂.₅ concentration decreases in the BTH2+26 cites from the 2016/17 heating season (HS1617) to the 2017/18 heating season (HS1718), we investigated the contributions of meteorological conditions and emission-reduction measures by Community Multi-Scale Air Quality (CMAQ) model simulations. The source apportionments of five sector sources (i.e., agriculture, industry, power plants, traffic and residential), and regional sources (i.e., local, within-BTH: other cities within the BTH2+26 cities, outside-BTH, and boundary conditions (BCON)) to the PM₂.₅ decreases in the BTH2+26 cities were estimated with the Integrated Source Apportionment Method (ISAM). Mean PM₂.₅ concentrations in the BTH2+26 cities substantially decreased from 77.4 to 152.5 μg m⁻³ in HS1617 to 52.9–101.9 μg m⁻³ in HS1718, with the numbers of heavy haze (daily PM₂.₅ ≥150 μg m⁻³) days decreasing from 17-77 to 5–30 days. The model simulation results indicated that the PM₂.₅ concentration decreases in most of the BTH2+26 cities were attributed to emission reductions (0.4–55.0 μg m⁻³, 2.3–81.6% of total), but the favorable meteorological conditions also played important roles (1.9–25.4 μg m⁻³, 18.4–97.7%). Residential sources dominated the PM₂.₅ reductions, leading to decreases in average PM₂.₅ concentrations by more than 30 μg m⁻³ in severely polluted cities (i.e., Shijiazhuang, Baoding, Xingtai, and Beijing). Regional source analyses showed that both local and within-BTH sources were significant contributors to PM₂.₅ concentrations for most cities. Emission controls in local and within-BTH sources in HS1718 decreased the average PM₂.₅ concentrations by 0.1–47.2 μg m⁻³ and 0.3–22.1 μg m⁻³, respectively, relative to those in HS1617. Here we demonstrate that a combination of favorable meteorological conditions and anthropogenic emission reductions contributed to the improvement of air quality from HS1617 to HS1718 in the BTH2+26 cities.

Both submerged macrophytes (SMs) and artificial macrophytes (AMs) have been widely used to improve water quality in eutrophic water. However, in heavily eutrophic aquatic ecosystems, the purification function of SMs is often restricted by the poor growth state due to competition from algae, while the purification function of AMs is often restricted by the limited carbon source supply for biofilm microbes attached to the AM surface. The objective of this study was to develop a new strategy to increase pollutant removal efficiency (RE) by combining the use of SMs and AMs. Pilot-scale microcosms, including treatments with both SMs and AMs (S&A), only SMs (SO) and only AMs (AO), were established to identify the performance of the new strategy. The results suggest that treatment S&A obtained REs of 88.9% for total nitrogen (TN) and 48.1% for chemical oxygen demand (COD); as comparison, treatments SO and AO obtained REs of 77.4% and 81.2% for TN and REs of −13.7% and 39.0% for COD, respectively. Compared with SO, the S&A treatment benefited SM growth in biomass, leaf chlorophyll concentration and root activity by inhibiting algae growth. In addition, compared with treatment AO, S&A increased the biofilm microbial biomass and the relative abundance of nitrifiers of families Nitrosomonadaceae and Nitrospira attached to AM surfaces. Therefore, by the mutual promotion of SMs and biofilms on AMs, the synergic application of SMs and AMs is a useful strategy for improving TN and COD REs in eutrophic water bodies such as rivers and constructed wetlands.A strategy was developed to increase nitrogen and COD removal in eutrophic water by the mutual promotion of submerged macrophytes and biofilms on artificial macrophytes.

The environmental-friendly photocatalytic process with a magnetic catalyst CoFe₂O₄/TiO₂ mediated by solar light for ibuprofen (IBP) degradation in pure water, wastewater effluent and artificial seawater was investigated systematically. The study aims to reveal the efficiency, the mechanism and toxicity evolution during IBP degradation. Hydroxyl radicals and photo-hole (h⁺) were found to contribute to the IBP decay. The presence of SO₄²⁻ showed no significant effect, while NO₃⁻ accelerated the photodegradation, and other anions including HCO₃⁻, Cl⁻, F⁻, and Br⁻ showed significant inhibition. The removal efficiency was significantly elevated with the addition of peroxymonosulfate (PMS) or persulfate (PS) ([Oxidant]₀:[IBP]₀ = 0.4–4), with reaction rate of 5.3–13.1 and 1.3–2.9 times as high as the control group, respectively. However, the reaction was slowed down with the introduction of H₂O₂. A mathematic model was employed to describe the effect of ferrate, high concentration or stepwise addition of ferrate was suggested to play a positive role in IBP photodegradation. Thirteen transformation products were identified and five of them were newly reported. The degradation pathways including hydroxylation, the benzene ring opening and the oxidation of carbon were proposed. IBP can be efficiently removed when spiked in wastewater and seawater despite the decreased degradation rate by 41% and 56%, respectively. Compared to the IBP removal, mineralization was relatively lower. The adverse effect of the parent compound IBP to the green algae Chlorella vulgaris was gradually eliminated with the decomposition of IBP. The transformation product C178a which possibly posed toxicity to rotifers Brachionus calyciflorus can also be efficiently removed, indicating that the photocatalysis process is effective in IBP removal, mineralization and toxicity elimination.

Bifenazate is a novel acaricide for selective foliar spraying and is widely used to control mites in agricultural production. However, its toxicity to aquatic organisms is unknown. Here, a zebrafish model was used to study bifenazate toxicity to aquatic organisms. Exposure to bifenazate was found to cause severe cardiotoxicity in zebrafish embryos, along with disorders in the gene expression related to heart development. Bifenazate also caused oxidative stress. Cardiotoxicity caused by bifenazate was partially rescued by astaxanthin (an antioxidant), accompanied by cardiac genes and oxidative stress-related indicators becoming normalized. Our results showed that exposure to bifenazate can significantly change the ATPase activity and gene expression levels of the calcium signaling pathway. These led to heart failure, in which the blood accumulated outside the heart without entering it, eventually leading to death. The results indicated that bifenazate exposure caused cardiotoxicity in zebrafish embryos through the induction of oxidative stress and inhibition of the calcium signaling pathway.

Wetland snakes, as top predators, are becoming globally recognised as bioindicators of wetland contamination. Livers are the traditional test organ for contaminant exposure in organisms, but research is moving towards a preference for non-lethal tissue sampling. Snake scales can be used as an indicator of exposure, as many metals bind to the keratin. We used laser ablation with inductively coupled plasma-atomic emission spectroscopy and mass spectrometry (LA-ICP-MS) to quantify the concentrations of 19 metals and metalloids (collectively referred to ‘metals’ hereafter) in Western tiger snake (Notechis scutatus occidentalis) scales from four wetlands along an urban gradient, and compared them to concentrations measured in captive tiger snake scales. We conducted repeat measures to determine the concentration accuracy of each metal using LA-ICP-MS. Concentrations in wild Western tiger snake scales were significantly higher than in reference tiger snake scales for most metals analysed, suggesting accumulation from environmental exposure. We compared the scale concentrations to sediment concentrations of sampled wetlands, and found inter-site differences between mean concentrations of metals in scales parallel patterns recorded from sediment. Four metals (Mn, As, Se, Sb) had strong positive correlations with liver tissue contents suggesting scale concentrations can be used to infer internal concentrations. By screening for a larger suite of metals than we could using traditional digestive methods, we identified additional metals (Ti, V, Sr, Cs, Tl, Th, U) that may be accumulating to levels of concern in tiger snakes in Perth, Western Australia. This research has progressed the use of LA-ICP-MS for quantifying a suite of metals available in snake scales, and highlights the significance of using wetland snake scales as a non-lethal indicator of environmental contamination.