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Phenolic content and antioxidant activity of hydromethanolic and aqueous extracts of aerial parts of Phlomis crinita. Полный текст
2022
Ismahane Derafa | Smain Amira | Fatima Benchikh | Walid Mamache | Chahrazed Kaoudoune
Phlomis crinita is a plant species of the family Lamiaceae including more than 100 perennial herbs, shrubs, and sub-shrubs species native to the Mediterranean, Central Asia, and India. This species is commonly a good natural source of various secondary metabolites. Therefore, the present study was conducted to determine phenolic content and antioxidant activity of hydromethanolic (PC ME) and aqueous extracts (PC AQE) of aerial parts of P. crinita. Total polyphenols, flavonoids and tannins were quantified, respectively by the methods of Folin-Ciocalteu reagent, aluminum trichloride (AlCl3) and Bate-Smith method. The in vitro antioxidant activity was assessed using DPPH and ABTS•+ radical scavenging, β-carotene-linoleic acid, reducing power and ferrous ion chelating activity assays. PC ME showed high level of tannins (132,13 ±0.68 µg TAE/mg extract) and total phenolic content (82.71±0.79 µg GAE/mg extract), in addition a marked inhibiting oxidation activity of β-carotene/ linoleic acid (74.10%) was observed. Results showed also a higher iron-chelating activity of PC ME (0.20 mg/mL) compared to PC AQE (0.046 mg/mL). The plant extracts revealed a significant antioxidant activity as evidenced by the DPPH and ABTS radical scavenging activity (IC50 = 0.103 mg/mL for PC ME and 0.144 mg/mL for PC AQE) for DPPH assay and (IC50 = 0.0130 mg/mL for PC ME and 0.0187 mg/mL for PC AQE) , as well as the PC ME exhibits higher reducing power (IC50 =0.288mg/mL) than PC AQE (0.296 mg/mL). As a result, P.crinita is suggested as a promising and effective therapeutic medicinal plant for the treatment of several diseases.
Показать больше [+] Меньше [-]Alkaline DMSO superoxide and radical scavenging, cupric reducing antioxidant capacity (CUPRAC) and polyphenol contents of aqueous and methanol extract from Achilllea santolinoides L. aerial parts Полный текст
2022
Soulaf Mehlous | Smain Amira | Fatima Benchikh | Hassiba Benabdallah | Walid Mamache | Chawki Bensouici | Hocine Laouer | Karima Loucif
The genus Achillea (Yarrow)is one of the most important medicinal plants. Nowadays, different medicinal functions of yarrow such as spasmolytic, choleretic, treatment of wounds and anti-inflammatory activities, make it as an important medicinal plant. The purpose of this study was to determine the antioxidant activity of aqueous (AAE) and methanol (AME) extracts from the plant Achillea santolinoides L. (A. santolinoides L.) aerial parts in vitro. Quantitative evaluation of polyphenols, flavonoids and tannins were determined using Folin-Ciocalteu reagent, aluminum chloride (AlCl3) and Bate Smith methods, respectively. Antioxidant activity was carried out using ABTS radical scavenging, alkaline DMSO superoxide radical scavenging and cupric reducing antioxidant capacity (CUPRAC). The obtained results showed that the highest content in total phenolic, flavonoids and tannins was found in the AME with values of 210.78±0.001 µg GAE/mg Dw, 21.18±0.025 µg QE/mg DW and198.73±0.014µg TAE/mg DW, respectively. For the in vitro antioxidant activity, AME had the strongest ABTS and DMSO alkaline radical scavenging activity (IC50= 6.74±0.16 µg/mL and 15.13±0.92 µg/mL, respectively) and the CUPRAC reducing with A0.50 of 76.56±2.35 µg/mL. The results of the present study confirm the use of the genus Achillea in the treatment of various diseases as a powerful antioxidant.
Показать больше [+] Меньше [-]In Vitro of Melaleuca viridiflora Sol. ex Gaertn Plant Investigation of Antimicrobial, Anticancer and Cytotoxic Activities Полный текст
2022
Eda Sönmez Gürer | Tutku Tunç
In this study; it was aimed to investigate the anticancer and cytotoxic activities on DU-145 prostate cancer and MCF-7 breast cancer cell lines and WI-38 human fibroblast cell line of essential oil (Nioli) obtained from Melaleuca viridiflora Sol. ex Gaertn. plant and its antimicrobial effects on various bacteria and yeast cells. Content analyzes of Nioli essential oil were made by Gas Chromatography-Mass Spectrometry (GK-KS). The antimicrobial effects of the essential oil were determined using Disk Diffusion and Minimum Inhibition Concentration (MIC) tests. Its cytotoxic effects were determined by the XTT (2,3-bis-(2-methoxy-4-) nitro-5)-sulfophenyl)-2H-tetrazolium-5-carboxanilide) test; were investigated at different concentrations in breast cancer (MCF-7), prostate cancer (DU-145) and healthy human fibroblast (WI-38) cell lines. In the disc diffusion method of essential oil; It was determined that it formed an inhibition zone against Klebsiella pneumoniae bacteria at 200, 100, 50, 25 and 12.5 µg/mL concentrations, and against Candida albicans yeast at 200 and 100 µg/mL concentrations. Nioli essential oil showed the highest antibacterial effect against Bacillus cereus. While it was moderately effective against Pseudomonas aeruginosa and Candida albicans, it reached MIC values effective against other bacteria. It was observed that the anticancer activity of Nioli essential oil was more effective in DU-145 prostate cancer cells compared to MCF-7 breast cancer cells, and it had no toxicity in WI-38 healthy human fibroblast cells.
Показать больше [+] Меньше [-]Yogurt as Probiotic: Comparative Effect on Growth Performance of Broiler Japanese Quail (Coturnix Japonica) Полный текст
2022
Md. Amir Hossain | Jannatul Mawa Momu
Yogurt is a natural source of gut-friendly bacteria. It has a considerable body of evidence that supports the significant positive effects of yogurt as probiotics on quail production performance and health. Yogurt containing bacteria can improve quail economic indexes and resistance to pathogens. With this objective, 240 growing Japanese quails were randomly distributed into three groups A1 (control), A2 (Yogurt), and A3 (Protexin) (4 replicates/treatment of 20 birds) to investigate the effect of yogurt as a probiotic source. Birds were allowed to be fed ad libitum with a commercial quail ration. Yogurt and Protexin were mixed at the rate of 5 ml/L (5ml into 1 lt. water) and 1g/L (1gm into 1 lt. water) in A2 and A3, respectively. Six (6) weeks of investigation showed a significantly higher result in yogurt than in the other two groups. Body weight had a significant difference between the control and protexin groups. Body weight gain (g) was significantly heavier in A2 at the finisher phase. No significant effect was observed in feed intake, but FCR (Feed Conversion Ratio) was significantly lower in A2, but no significant effect was noticed between A1 and A3. The mortality percentage was higher in the control group (2.66%) and lowest in yogurt (1.03%). Carcass characteristics were significantly heavier in A2 than A1 and A3. Non-carcass characteristics illustrated significant differences among the three groups, but a non-significant difference was observed in head weight. The gross return per bird was higher in A2 (12.05 BDT), whereas it was 6.55 BDT and 7.08 BDT for A1 and A3. With those observations, it can be concluded that yogurt successfully enhanced overall broiler performance and gross return of Japanese quail.
Показать больше [+] Меньше [-]Efficacy of Ammonization to Eliminate Common Mycotoxins Полный текст
2022
Veysel Doğan | Armağan Hayırlı
Mycotoxin is a worldwide problem threatening animal health and performance as well as public health. The objective of this experiment was to test the effect of ammonization on elimination of common mycotoxins in laying hen compound feed (CF) and dairy cattle total mixed ration (TMR). The CF for laying hens and TMR for dairy cows were contaminated with commonly occurring mycotoxins [aflatoxin B1 (AFB1), ochratoxin A (OTA), and zearalenone (ZEA)] at 25 times of their accepted legal limits (20 ppb, 200 ppb and 500 ppb, respectively). They were then subjected to ammonization with ammonium bicarbonate (NH4HCO3) at 50C for 24 hours under the atmospheric pressure. Mycotoxin levels were analyzed using the LC-MS/MS technique. The elimination levels in CF and TMR were 53% and 54% for AFB1; 31% and 31% for OTA and 22% and 22% for ZEA, respectively. In conclusion, ammonization was effective in destroying common mycotoxin, at an order of AFB1 > OTA > ZEA.
Показать больше [+] Меньше [-]Some Carcass Quality Characteristics of Anatolian Buffaloes Slaughtered at a Private Slaughterhouse in Samsun Province Полный текст
2022
Sena Camcı | Hüseyin Erdem
In this study, it was aimed to reveal some carcass quality characteristics of 60 head Anatolian water buffalo slaughtered in a private slaughterhouse in Samsun. Between February and May 202, information about the animals and their fattening status were obtained from the owners of the animals that came for slaughter on the days when the slaughter was intense. Hot carcass weight, marbling score, back fat thickness, Longissimus dorsi muscle depth and width, muscle pH, muscle and fat color were determined in slaughtered animals. Scales developed for this purpose were used for marbling score and color of muscle and fat, and pH was determined using a pH meter for meat. In the study, it was determined that the age of the slaughtered animals did not affect the carcass quality characteristics, while the fattening period was found to be effective on the carcass weight (P
Показать больше [+] Меньше [-]The Effect of Irrigation Water Quality and Leaching Ratio on Some Yield Parameters in Alfalfa (Medicago Sativa L.) Полный текст
2022
Sertan Avcı | Engin Yurtseven
This study was carried out over the experimental fields of Ankara University, Faculty of Agriculture Gümüşdere Campus. Experiments were conducted with 3 different irrigation water salinity levels (S1=0.25 dS/m – control/tap water, S2=1.5 dS/m, S3=3.0 dS/m) and 4 different leaching ratios (LF1=10%, LF2 =20%, LF3 = 35%, LF4=50%) in randomized plots factorial experimental design with 3 replications. Totally, 36 (3×4×3) lysimeters were used in present experiments. Plant height, fresh and dry herbage yield and total ash content of alfalfa plants were analyzed. With increasing salinity levels, plant height, fresh and dry herbage yields decreased and total ash contents increased. Positive effects of leaching applications were observed, but this effect was not found to be significant. The main reason for this situation may be the necessity of a good drainage system for a good leaching application.
Показать больше [+] Меньше [-]The extent of Vulnerability to Food Insecurity and Household Coping Strategies: Case of Yam Farmers in Ekiti State, Nigeria Полный текст
2022
Ayoola Ibukun Ogunyemi | Adewale Isaac Olutumise | Ademola Adegoroye
The study analysed the extent of vulnerability to food insecurity and household coping mechanisms among yam farmers in Ekiti State, Nigeria. Cross-sectional data were used for this study with the aid of a well-designed questionnaire. A multistage sampling procedure was used to select 360 respondents. Multinomial logit (MNL) model and Feasible Generalized Least Square (FGLS) method were employed for the data analysis. The results of the FGLS model showed that 49.3% of the households were food secure and experienced low vulnerability to food insecurity. However, 30.23% of them were food insecure and highly vulnerable; they are considered as chronically food-insecure households. Also, the study revealed that 11.01% of the food secure households may be food insecure in the future if necessary attention and intervention are not given by both households and the government. Again, 9.4% of the households that were experiencing food shortage, as at the time of the study, may recover in the future. The findings of MNL revealed that the age of the household head, main occupation, household size, land size, net household income, and membership in a cooperative society were the main significant factors in yam farming households' decision to use coping strategies. As a result, it is recommended that leveraging the potential role of coping mechanisms already used by households during food shortages, and building a comprehensive human capital development, such as education, will be vital policy options to reduce food insecurity.
Показать больше [+] Меньше [-]Investigation of Drying Kinetics and Powder Product Properties of Dragon Fruit Powders Dried by Microwave Assisted Foam Drying Method Полный текст
2022
Kadriye Altay
In this study, dragon fruit was dried by a microwave-assisted foam drying method and the effect of different microwave powers (350, 460 and 700W) on the drying kinetics and powder product properties were investigated. The properties (foam stability, expansion and density) of the foams obtained by adding egg white to dragon fruit puree were examined. The highest R2 and lowest RMSE and χ2 values of foam dried samples obtained from the Silva models. The effective moisture diffusion coefficient values of the samples (5.93×10-8-1.16×10-7 m2/s) increased due to the increase of microwave power. Activation energy values were calculated as 74.77 W/g. The effects of different microwave powers on moisture, water activity, color and powder product properties of dragon fruit powders dried by foam drying method were determined. In addition, in all conditions where microwave foam drying is performed, dragon fruit powders showed very good flowability and low stickiness.
Показать больше [+] Меньше [-]Bioremediation of Heavy Metals by Use of Bacteria Полный текст
2022
Orcan Demircan | Abdul Razaque Memon
Heavy metal pollution generally occurs due to socio-economic, industrial, and anthropogenic activities, which may cause an environmentally hazardous and serious severe threat to the survival of the organisms (genotoxic, carcinogenic, and clastogenic effects on it). Many physical and chemical remediation approaches have been proposed to deal with this pollution, but these are very time-consuming and costly. While bioremediation stands out as an inexpensive and efficient approach, the use of bacteria is thought to be a potential and productive organism to prevent this pollution. This review has evaluated the bacterial potential to clean up heavy metals from the environment and elucidated the mechanisms responsible for bioremediation.
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