While shellfish species are widely consumed due to their nutritional advantages, they are also among the top eight food items for food-borne allergies. Five distinct thermal processing techniques were applied to the crustacean to investigate the tropomyosin level variations caused by heat processing. Fresh shrimp and prawns were utilized as controls for the determination of allergen-encoding genes. Prior to molecular analysis, the proximate composition and acidity of raw and processed samples were also performed. The yield and purity of DNA were also determined. Melting curve and gel electrophoresis tests verified the existence of allergen-coding genes. Thermal processing procedures affected the proximate composition, particularly the total protein and fat concentrations, according to the findings. Following the heat treatment, the pH levels decreased, particularly in the grilled samples. There were also significant differences in the quantity and quality of the extracted DNA. Regardless of crustacean species, the tropomyosin-encoding gene was detected in both fried and grilled samples. These findings demonstrated that RT-PCR identification and validation of the crustacean allergy gene by gel electrophoresis might be a reliable approach for the thermally treated shrimp and prawn samples. This study shows that investigating the allergen coding gene might provide a viable way for detecting food-borne allergens in other thermally processed food items, which are becoming more concerned about food safety.

En un invernadero del Centro Internacional de Agricultura Tropical, CIAT, Cali (Colombia) se evaluó la aplicación de cinco inóculos de hongos micorrícico arbusculares, HMA: Kuklospora colombiana, Gigaspora margarita, Glomus manihotis y la mezcla de estos con y sin esterilización, en sustrato sin desinfectar (SD) y desinfectado (D) sobre variables de rendimiento (biomasa aérea y radical, longitud radical), colonización por HMA y concentración foliar de nutrientes en la gramínea Melinis minutiflora Beauv., con el objetivo de seleccionar los inóculos más eficientes. Se utilizaron como unidades experimentales materos de 13.5 x 16.0 x 14.0 cm. El sustrato empleado fue suelo procedente de un Inceptisol con baja disponibilidad de nutrientes, tamizado y mezclado con arena. Los inóculos de Gi. margarita y Gl. manihotis presentaron los mejores resultados en la acumulación de biomasa aérea y radical, longitud radical, porcentaje de colonización micorrícica y concentración de elementos. Kuklospora colombiana presentó efectos inhibitorios sobre las variables evaluadas. La condición del sustrato SD favoreció la acumulación de biomasa aérea y radical y la concentración de fósforo (P) en la biomasa aérea, además, estimuló la longitud radical de M. minutiflora. La concentración de N, K, Ca y Mg en la biomasa aérea fue mayor en el sustrato D. Los resultados muestran que Mellinis minutiflora con inoculación HMA es promisoria para la recuperación de suelos degradados. | The effect of five inocula of arbuscular mycorrhizal fungi (AMF) on the grass Melinis minutiflora Beauv. was investigated under greenhouse conditions at the International Center for Tropical Agriculture (CIAT), Cali, Colombia, with the aim of selecting the most efficient AMF inocula. Non-disinfected (ND) and disinfected (D) substrates were studied. Inocula were: Kuklospora colombiana, Gigaspora margarita, Glomus manihotis and a mixture of those three species of AMF with and without sterilization. Yield parameters were aerial and radical biomass, root length, mycorrhizal colonization and N, P, K, Ca, and Mg concentrations in the aerial biomass. Pots measuring 13.5 x 8.0 x 14.0 cm were used as experimental units. Inceptisol soil, with low nutrient availability, previously sieved and mixed with sand was used as substrate. Gi. margarita and Gl. manihotis inocula showed the best results in the accumulation of aerial and root biomass, root length, mycorrhizal colonization and concentration of elements in the aerial biomass, while Ku. colombiana presented inhibitory effects on the variables evaluated. ND substrate condition increased accumulation of aerial and radical biomass and P concentration in the aerial biomass, also stimulated root length of M. minutiflora. Aerial biomass had higher concentrations of N, K, Ca, and Mg in D substrate. Mellinis minutiflora is a promising grass species for rehabilitation of degraded soils in combination with arbuscular mycorrhizal fungi inoculation.

This study aimed to evaluate the biochemical and nutritional profiles of three cauliflower cultivars—Valentena, Carotena, and Snow White—focusing on chlorophyll, carotenoids, anthocyanins, vitamin C, flavonoids, phenolics, and antioxidant activity. Uniform curds were harvested at 60 days post-sowing from Baratia, Dumuria, Khulna, and analyzed at Khulna Agricultural University. Valentena exhibited the highest chlorophyll content (40.06±0.39 µg/100g FW chlorophyll a, 28.98±3.35 µg/100g FW chlorophyll b), superior lycopene (8.71±0.38 µg/100g FW) levels. Carotena showed the highest total carotenoid content (60.52±1.76 µg/100g FW) and β-carotene (26.99±0.44 µg/100g FW), while Snow White had the lowest values across most parameters. Valentena also led in anthocyanins (101.56±3.9 mg/L FW) and total flavonoids (79.56±10.36 mg/100g FW), with Carotena having the highest vitamin C content (60.05±2.93 µg/g FW). DPPH assays indicated that Valentena showed the most effective antioxidant (IC50 = 43.65±3.56 mg/mL FW), followed by Carotena and Snow White. Hierarchical clustering and pricipal component analysis (PCA) revealed distinct biochemical profiles: Valentena and Carotena shared similarities in carotenoids and antioxidant activity, whereas Snow White differed significantly. Linear discriminant analysis identified lycopene, chlorophyll b, and β-carotene as major differentiators, highlighting the diverse nutritional and antioxidant properties of these cauliflower varieties. The findings highlight the potential of Carotena and Valentena for health-conscious consumers seeking nutrient-rich, antioxidant benefits in functional meals.

The paper presents tests connected to the torrefaction of agro-biomass residues as a case of biomass valorisation. The aim of the work is to compare the changes in energy and chemical properties of millet stalk (Panicum miliaceum L.) before and after the torrefaction process. The torrefaction of the millet stalk was done by using a scale reactor in two temperatures, 275oC and 300oC, in an N2 atmosphere. The millet stalk torrefied at 300oC has more promising parameters, i.e., higher heating value HHV 24,57 MJ/kg, the content of carbon 64,90% and energy density 1,42 compering to biochar produced at 275oC - 22,57 MJ/kg, 60,90% and 1,31 respectively. The results showed that torrefaction improves the parameters of the millet stalk for higher-quality biofuel, which can be used for heat generation.

Hungary and Turkey have a considerable share in world wheat markets. In 2018, Hungary’s export value of wheat and wheat flour reached to 411 million euros and it formed 32 per cent of overall cereal & milling industry export value of the country. Export value of Turkey for the same commodities was 875 million euros in the same period and it formed 77 per cent of total cereal & milling industry export value (International Trade Centre, 2019). Considering their potential and recent upward trends in trade, this study aims to determine the competitiveness of the wheat sectors in Hungary and Turkey. The study examines the competitiveness level of both countries by using Balassa’s Revealed Comparative Advantage index and Michaely index. The examination range covers the 10-year period between 2009 and 2018. Majority of data in this work was obtained and derived from International Trade Centre database. According to analysis results, Hungary has a higher degree of specialization vis-a-vis Turkey.

The oleaster (Elaeagnus angustifolia L.), also known as wild olive, is a small fruit with three parts: the outer peel or exocarp layer, the edible part or mesocarp layer, and the inner seed or endocarp layer. The mesocarp layer is rich in essential vitamins and has great potential for use in various food products. The flour made from the mesocarp layer has a moisture content of 8.99%, an ash content of 2.66%, a fat content of 0.55%, a protein content of 5.99%, a crude fiber content of 3.32%, and a total dietary fiber (TDF) content of 26.36%. The TDF content is divided into insoluble dietary fiber (IDF) and soluble dietary fiber (SDF), which are 21.35% and 5.01%, respectively. The flour has color values of L*: 75.14, a*: 2.86, b*: 23.87, and a water activity value of 0.314. The water solubility, water absorption, and oil absorption are 67.33%, 4.91 g water/g sample, and 2.26 g oil/g sample, respectively. Additionally, the mesocarp layer contains minerals such as Mg, P, K, Ca, Fe, and Na. The mesocarp layer significantly affected the thermomechanical properties of wheat flour. As the substitution level of the mesocarp layer increased from 10 to 30%, the water absorption capacity, dough development time and stability time of the wheat dough significantly decreased. Specifically, the water absorption capacity dropped from 53.5% to 47%, dough development time reduced from 1.10 to 0.75 min, and stability time decreased from 8.90 to 2.25 min. Substituting a mesocarp layer in wheat flour can significantly improve product shelf-life due to slower retrogradation. The mesocarp layer is an functional ingredient in the food industry.

The purpose of the scientific article is the attempt of determining the level of human capital of persons from the oldest age groups who live in rural areas of Southeastern Poland. The selection of research topic has a relationship with the demographic changes which take place both in Poland and in the whole Europe. They bring about a number of changes which affect almost all areas of life of modern societies. The research was conducted in 2015 on a sample of 150 people over the age of 60 in the counties of Nowy Targ and Myślenice located in the Małopolska Voivodeship. The level of human capital of the surveyed seniors can be described as relatively low. The structure of education, occupational and educational activity (in the field of increasing knowledge and acquiring new skills), moreover self-assessment of their health status and taking actions aimed at improving it, for example through physical activity, confirm it. | Celem opracowania jest próba określenia poziomu kapitału ludzkiego osób z najstarszych grup wieku zamieszkujących na obszarach wiejskich Polski południowo - wschodniej. Wybór tematu badań miał związek z przemianami demograficznymi, dokonującymi się zarówno w kraju jak i w całej Europie. Pociągają one za sobą szereg zmian dotykających niemal wszystkich dziedzin życia współczesnych społeczeństw. Badania zostały przeprowadzone w 2015 roku na próbie 150 osób w wieku 60+ na terenie powiatów nowotarskiego i myślenickiego położonych w województwie małopolskim. Poziom kapitału ludzkiego badanych seniorów można określić jako stosunkowo niski. Świadczą o tym na przykład: struktura wykształcenia, aktywność zawodowa oraz edukacyjna (w zakresie poszerzania wiedzy oraz zdobywania nowych umiejętności), a także samoocena swojego stanu zdrowia i podejmowanie działań mających na celu jego poprawę np. poprzez aktywność fizyczną. | kapitał ludzki, obszary wiejskie, osoby starsze, proces starzenia się | human capital, rural areas, elderly person, aging process | 13 | 116-125 | 2

The purpose of the scientific article is the attempt of determining the level of human capital of persons from the oldest age groups who live in rural areas of Southeastern Poland. The selection of research topic has a relationship with the demographic changes which take place both in Poland and in the whole Europe. They bring about a number of changes which affect almost all areas of life of modern societies. The research was conducted in 2015 on a sample of 150 people over the age of 60 in the counties of Nowy Targ and Myślenice located in the Małopolska Voivodeship. The level of human capital of the surveyed seniors can be described as relatively low. The structure of education, occupational and educational activity (in the field of increasing knowledge and acquiring new skills), moreover self-assessment of their health status and taking actions aimed at improving it, for example through physical activity, confirm it. / Synopsis. Celem opracowania jest próba określenia poziomu kapitału ludzkiego osób z najstarszych grup wieku zamieszkujących na obszarach wiejskich Polski południowo - wschodniej. Wybór tematu badań miał związek z przemianami demograficznymi, dokonującymi się zarówno w kraju jak i w całej Europie. Pociągają one za sobą szereg zmian dotykających niemal wszystkich dziedzin życia współczesnych społeczeństw. Badania zostały przeprowadzone w 2015 roku na próbie 150 osób w wieku 60+ na terenie powiatów nowotarskiego i myślenickiego położonych w województwie małopolskim. Poziom kapitału ludzkiego badanych seniorów można określić jako stosunkowo niski. Świadczą o tym na przykład: struktura wykształcenia, aktywność zawodowa oraz edukacyjna (w zakresie poszerzania wiedzy oraz zdobywania nowych umiejętności), a także samoocena swojego stanu zdrowia i podejmowanie działań mających na celu jego poprawę np. poprzez aktywność fizyczną.