High vacuum steam distillation, simultaneous distillation extraction, steam distillation, purge and trap methods are used for sample preparation in volatile component analysis. Since these methods are difficult and costly to implement, these methods have left their place to the solid phase microextraction technique. Extraction conditions such as extraction temperature, extraction time, sample volume and fiber type should be optimized in order to increase the efficiency of extraction in volatile component analysis with solid phase microextraction in foods. In the optimization of extraction conditions in volatile component analysis of foods, studies have been carried out to utilize the response surface method, which reduces the number of experiments and also examines the interaction effect of the independent variables. In this review, it is aimed to give information about current studies using response surface methodology in optimization of volatile compound analysis of foods using solid phase microextraction technique.

This study assessed seasonal incidence, economic losses, the efficacy of therapeutic protocols, the recovery time of affected animals and specific prophylactic measures applied to sheep with acute infectious pododermatitis. The studies were conducted over a period of 12 months in 3 different sheep farms from private units in the same area. The results of the study showed an increased incidence of the disease in all 3 farms, with an average of 26.94% of the sheep flock. The incidence of the disease was increased in the months of April-May-June-July and September-October (30%), when there were heavy rains. The high morbidity led to economic losses through the decrease in milk production by approximately 30% and the decrease in the weight of the sheep by 10.58% (4.2 kg) of their normal weight. The therapeutic protocol applied locally as well as parenterally, combined with a foot bath with 10% zinc sulphate solution, were effective in treating acute infectious pododermatitis of sheep. The average recovery time (days) was approximately the same in the three groups of sheep (5.25 ± 0.68 days for cases with moderate diseases and 10.2 ± 0.22 for cases with severe diseases).

Investigate the changes that occur in the placenta tissues of pregnant rats that were administered acrylamide (AA) and vitamin E as a protective agent during pregnancy. Thirty rats that were proven positive for pregnancy with vaginal smear test were randomly distributed into control, corn oil, vitamin E, acrylamide and vitamin E + acrylamide groups. Pregnant rats were decapitated on the 20th day of the experiment. Malondialdehyde (MDA), reduced glutathione (GSH), total antioxidant capacity (TAS), total oxidant capacity (TOS) and Xanthine oxidase (XO) levels were measured in placenta tissues. It was determined that acrylamide application during pregnancy statistically significantly increased MDA, TOS and XO levels and reduced GSH and TAS levels in the placenta tissue of pregnant rats when compared to all other groups, and GAS and TAS levels statistically significantly increased in vitamin E administered group when compared to all other groups and TOS and XO levels were decreased to control group levels. It was observed that orally administered AA changed the antioxidant / oxidant equilibrium favoring the oxidants by increasing MDA, XO and TOS levels in pregnant rats and caused oxidative stress, while vitamin E administration returned the antioxidant / oxidant equilibrium back to normal levels, preventing oxidative stress induced toxicity.

Dornelor Basin is characterised by numerous high quality water sources, which is proven by the fact that the main mineral waters on the Romanian market come from this area. The study aimed to provide data on the occurrence and human infective potential of Giardia and Cryptosporidium, as the most important water-borne parasites, from Bistrița river tributaries of Dornelor basin, North-Eastern Romania. Water samples were collected from 10 tributaries of the Bistrita river, from the level of sampling stations set upstream and downstream from the anthropic communities. The harvested water samples were further processed using nonmolecular methods in order to isolate (oo)cysts. Subsequently, the isolated Cryptosporidium and Giardia (oo)ccyst were molecularly characterized through PCR and genomic sequencing, which led to the identification of Giardia in order to identify them at species level. The outcomes revealed the fact that the waters of the emissaries under study have a low parasite load and that, upstream from the human settlements, the water is highly pure when related to the protozoa under study. The increased load of Giardia spp. and Cryptosporidium spp.

In this study, it was aimed to determine the approaches of primary school students’ parents participating in the school milk programme in the Isparta province. The data obtained by the questionnaire method were used from 359 students’ parents who were included in the school milk programme. The families were divided into four groups according to the household income. It was determined that the families consumed more pasteurized milk. The average age of the interviewed individuals was 37.14 years. About 36.21% of the parents were high school graduates. 53.48% of them spend money on milk less than 50 TL. Household size was 4.05 persons. 21.84% of them consume milk hot. The attitude of the parents to the school milk programme was generally positive (82.17%). The school milk programme was believed to be a habit of consuming milk. It was found that 33.33% of the surveyed parents were negative about the school milk programme because they did not trust the content of the milk distributed. In the study, 49.30% of the parents thought that the school milk programme increased the milk consumption habits of the children and 67.97% thought that there was not more supply of milk distributed. For the purpose of implementing the school milk programme, 48.75% of the families had a judge to love the milk and make milk drinking habit. The majority of the families (62.40%) were preferred daily milk in the products distributed and 44% believed that drinking milk was subjected to nutritional analysis. It is suggested that the Ministry of National Education and the Ministry of Health should inform families about the benefits of milk and school milk programme.

Agriculture is the second, after energy sector, emitter of greenhouse gasses (GHG), of which increased concentrations in the atmosphere are caused by human activities. In order to reduce GHG, parties ratifying the Kioto protocol have committed to prepare annual emission reports and pledged to reduce emissions. The aim of the study was to analyse changes of agricultural emissions in the World, the European Union (EU) and Poland in 1990-2014. The research uses the United Nations Food and Agricultural database (FAOSTAT), United Nations Framework Convention on Climate Chang (UNFCCC) and World Resources Institute (CAIT) databases. The analysis shows that in the World, in the examined period the total GHG emissions increased by 85%, whereas in agriculture by 15%. However, the EU as a member of Annex I parties had reduced total and agricultural emissions by 24% and 23%, respectively. The reduction of emissions was the result of the implementation of pro-environmental regulations. / Synopsis. Rolnictwo jest drugim po sektorze energii emitentem gazów cieplarnianych (GHG), których stężenie w atmosferze wzrasta w wyniku działalności człowieka. W celu ograniczenia emisji GHG kraje ratyfikujące porozumienie z Kioto zobowiązały się do sporządzania rocznych raportów emisji oraz do ich redukcji. Celem badań była analiza zmian wielkości emisji z rolnictwa na świecie, w Unii Europejskiej (UE) i Polsce w latach 1990-2014. W badaniach wykorzystano bazę danych Organizacji Narodów Zjednoczonych do Spraw Wyżywienia i Rolnictwa (FAOSTAT), Ramowej Konwencji Narodów Zjednoczonych w sprawie zmian klimatu (UNFCCC) oraz Światowego Instytutu Zasobów (CAIT). Z przeprowadzonej analizy wynika, że w badanym okresie na świecie nastąpił wzrost emisji GHG ogółem o 85%, a w rolnictwie o 15%. Jednakże UE należąca do grupy krajów rozwiniętych tj. Aneksu I obniżyła emisje ogółem i z rolnictwa odpowiednio o 24 i 23%. Redukcja emisji była efektem wdrażania pro środowiskowych regulacji prawnych.