Antigenic analysis of Peptococcus indolicus by crossed immunoelectrophoresis [bovines]
1980
Smyth, C.J. | Zimmerman, E.K. | Schwan, O. (University of Agricultural Sciences, Uppsala (Sweden). College of Veterinary Medicine, Dept. of Bacteriology and Epizootology)
Quantitative immunoelectrophoretic methods have been used to study the antigenic mosaic of Peptococcus indolicus, an anaerobic coccus frequently isolated from udder secretions from heifers and dry cows with mastitis. Thee antigenic components of liquid cultures of this bacterium were analyzed, compared and characterized, namely concentrated culture filtrate containing extracellular antigens, a cytoplasmic antigen fraction obtained by freeze-press disruption of bacterial cells and Triton X-100-soluble antigens from cell wall-membrane fractions. The extracellular antigens were further investigated because they proved to be particularly useful in preliminary studies on the antibody response of cows to P. indolicus. The possible cross-reactivity of peptococcal antigens with extracellular antigens from other bacteria causing, or associated with, mastitis was investigated. The contribution of medium components to the immunoprecipitate profile, the heat-stability of antigens and the relationship of serotypic antigens to those in the standard extracellular concentrate were established using co-immunoelectrophoresis with intermediate gel. Attempts to identify enzyme-active immunoprecipitates with histochemical enzyme staining methods revealed only glutamate dehydrogenase.
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