Enhancement of pollen embryo formation in Datura innoxia by charcoal [growth medium, agar, nutrient medium, anther culture]
1980
Tyagi, A.K. | Rashid, A. | Maheshwari, S.C. (Delhi Univ. (India). Dept. of Botany)
In recent years liquid medium has been shown to be better than agar-gelled medium for production of haploids by anther culture. However, on addition of charcoal to agar medium the anther response in Datura innoxia Mill, increases dramatically and is better than in liquid medium. For anthers with pollen at the premitotic stage, the best result was observed with 1 per cent charcoal in Difco agar and 1.5 per cent in Normal agar. The effect is possibly due to adsorption of substances inhibitory to androgenesis and emanating from anthers, as well as to substances present in the nutrient medium and agar.
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