Genotype, ploidy and physiological state in relation to isoperoxidases in Nicotiana [ tobacco, ploidy level, Nicotiana sylvestri, x Nicotiana tomentosiformis hybrid, Nicotiana tabacum]
1981
Trinh, T.H. | Van, K.T.T. | Marcotte, J.L. (Centre National de Recherche Scientifique (CNRS), 91 Gif-sur-Yvette (France). Lab. du Phytotron) | Gaspar, T.
Peroxidase and isoperoxidase patterns of tobacco have been comparatively analyzed in relation to parental origin and ploidy level (including hypohaploidy status) at different ages and physiological states. The interspecific hybrid N. sylvestris x N. tomentosiformis shows an additive pattern of parental peroxidases, which also resembles that of N. tabacum cv. Wisconsin and cv. Samsun. Enzyme activity (on dry weight and protein bases) decreases from the diploid to the hypohaploid forms of Wisconsin and Samsun with respective quantitative differences in the zymograms, when the plants are compared at the same chronological and vegetative stages. Abnormally vegetatively ageing hypohaploids recover all peroxidasic isoenzymes and reach an enzyme activity as high as those of haploids and diploids. Flowering hypohaploids, however, generally behave like haploids and diploids in changing their balance between acidic and basic peroxidases.
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