Acyl and pigment lipid composition of two chlorophyll-proteins [spinach thylakoids, acyl lipids]
1980
Rawler, A. | Henry, L.E.A. | Siegenthaler, P.-A. (Neuchatel Univ. (Switzerland). Lab. de Physiologie Vegetale et Biochemie)
A differential distribution of acyl and pigment lipids among chlorophyll-proteins of spinach, obtained by SDS-polyacrylamide gel electrophoresis of solubilized thylakoids, was demonstrated by thin-layer chromatography. Light-harvesting chlorophyll a/b-protein 2 and P-700 chlorophyll a-protein 1 were isolated and analysed for their lipid class composition including the fatty acid distribution within each lipid class. The light-harvesting chlorophyll a/b-protein 2 was rich in galactolipids and phosphatidylglycerol containing a high amount of 3-trans-hexadecenoic acid. In contrast, the P-700 chlorophyll a-protein 1 lacked these two lipid classes but was associated with sulfoquinovosyldiglyceride and phosphatidylinositol containing a comparatively large proportion of saturated fatty acids. Beta-carotene was preferentially associated with the chlorophyll a-protein whereas xanthophylls were restricted to the chlorophyll a/b-proteins. The present data support lateral heterogeneity in the distribution of pigment and acyl lipids within the photosynthetic lamellae.
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