Mitosis in Elaeis guineensis Jacq. [Oil Palm] race Deli Dura
1982
Imam, M.M.
Elaeis guineensis is very difficult cytological material to work with. Its chromosomes are smaller and have the tendency to stick together. The karyotype was analysed to produce a working model of Elaeis guineensis idiogram for standard use, against which all future cytological preparations of hybrid seedlings and plantlets produced through tissue culture could be matched to observe chromosomal anomalies. The seedlings were pretreated with two dosages of colchicine of strength 0.004% and 0.005% at different time intervals of 24 hours and 48 hours. Its roots were then washed in carnoy solution for 24 hours, stored in 70% ethyl alcohol in a fridge at -4 degrees C temperature. Results showed that 0.004$ concentrate yielded more dividing cells and pretreatment of 48 hours was more effective in producing better chromosomes. Fixation of root-tips were best at 6.30 a.m. or earlier when more prophase were observed. Metaphase was the most prevalent mitotic stage followed by anaphase, telophase and prophase. Further research with other suitable chemical would also be carried out
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