Isolation and identification of mycobaction dependent acid-fast bacteria (M. paratuberculosis) from bovine fecal material
1984
Jeon, Y.S. (Seoul National Univ., Suwon (Korea R.). Coll. of Veterinary Medicine) | Lee, B.W. (Chunnam National Univ., Kwangju (Korea R.)) | Kim, J.K. (Green Cross Veterinary Products Co., Ltd., (Korea R.))
Fecal material from cattle, which was confirmed to be infected with Johne's disease by clinical and pathological symptoms, was decontaminated with 4% NaOH and inoculated into the Lowenstein-Jensen media supplemented with 1% of heat-killed Mycobacterium bovis. After 2-4 week-incubation at 37 deg C, typical acid-fast mycobacteria was isolated. The requirements of mycobactin for growth and the other biochemical properties, isolated mycobacteria was identified as Mycobacterium paratuberculosis
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