Isolation of Aspergillus fumigatus and properties of its enzyme for Rhodotorula glutinis cell wall
1984
Pan, J.G. | Lee, J.S. (Korea Advanced Inst. of Science and Technology, Seoul (Korea R.). Dept. of Biological Science and Engineering)
A fungus producing cell wall lytic enzyme for Rhodotorula glutinis was isolated from local soil and identified partially as a species of Aspergillus fumigatus group. The cell wall lytic enzyme was an inducible exoenzyme and composed of at least lytic polysaccharidase and protease which act cooperatively in the lysis of intact cells. The lytic polysaccharidase was not able to hydrolyze beta 1,3 and beta 1,6-glucan which have the same types of bond as found in the cell wall of Ascomycetous yeasts. The lytic polysaccharidase alone was sufficient to hydrolyze the fractionated cell wall (alkali-insoluble residues) of R. glutinis, whereas it showed low activity against intact cells
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