Cloning and transcription of Escherichia coli cell division gene, sep
1984
Lee, M.J. (Seoul National Univ. (Korea R.). Cancer Research Inst.) | Walker, J. (Texas Univ. (USA). Dept. of Microbiology)
Sep gene which was one of the cell division genes coding for penicillin binding protein 3 was subcloned from lambda 607sep2 to plasmid pBR322. To maximize the expression of sep gene, it was also cloned to plasmid pLJ3 which has a strong promotor such as lac UV5 (lacP). It was confirmed that the sep gene cloned to pLJ3 was in the proper orientation for expression from lactose promotor. To analyze the expression efficiency of sep gene within the plasmids newly constructed, sep mRNA was assayed by using lambda 607sep2 DNA as a probe. Sep mRNA level increased 25 times in the cells carrying sep gene cloned to pBR322 compared to E. coli C600 which has wild type sep gene within the chromosome instead of plasmid
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