Purification and characterization of raw starch digesting enzyme from rhizopus oryzae
1986
Kim, C.J. | Oh, M.J. | Lee, J.S. (Chungnam Univ., Taejon (Korea R.). Dept of Food Science and Technology)
A raw starch-digesting enzyme from Rhizopus oryzae was purified by ammonium sulfate fractions, DEAE-sephacel column chromatography and Sephadex G-150 gel filtration. The specific activity of purified enzyme was 45.2 U/mg protein and the yield was 16.2%. The purified enzyme was found to be homogeneous by polyacrylamide gel electrophoresis and its molecular weight was estimated to be 67,000 by SDS-polyacrylamide gel electrophoresis, and also the enzyme had Km value of 4.082 mg/ml for raw corn starch. The optimal temperature and pH for the enzyme activity were 50 deg C and 4.5-5.0, respectively. Reaction product of raw corn starch by purified enzyme was glucose mainly
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