Polyphenoloxidase from starfruit (Averrhoa carambola, L.)
1986
Adnin bin Adnan, Tengku | Augustin, M.A. | Hasanah Mohd Ghazali (Universiti Pertanian Malaysia, Serdang, Selangor. Jabatan Sains Makanan)
Polyphenoloxidase (PPO) was isolated from starfruit (Averrhoa carambola, L.) and purified by acetone precipitation and dialysis. The optimum pH for the acetone purified PPO catalysed oxidation of catechol was found to be pH 7.2. Heat inactivation studies showed that the enzyme was heat-labile. Half-lives for PPO inactivation at 70 degrees C and 75 degrees C were found to be 4.6 min and 3.1 min respectively The Michaelis constant (K(,m)) and V(,max) for catechol with PPO was 0.061 M and 20 U ml-1 respectively
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