Effect of some media components and organic additives on callus induction in rice anther culture [Philippines]
1985
Zapata, F.J. | Torrizo, L.B. (International Rice Research Inst., Los Banos, Laguna (Philippines). Tissu Culture Facility)
In the N content experiment, the E10 control had optimum concentration for inducing maximum anther-producing calli. NH4+ was added to E10 medium as 134 mg (NH4+)2SO4/litre and NO3 was added as 2500 mg KNO3/litre. Adding mannitol at 1% stimulated the most anthers and induced almost 1.5 times as many calli as the control. Although not considered as a readily available C sources, mannitol may influence the medium's osmotic potential, a critical factor in callus induction. Coconut water contains myo-inositol, growth regulators, and amino acids, which can increase callus production. Adding 10% coconut water produced 1.4 times the calli in the control. Mashed banana increases plantlet formation in immature orchid embryos, but inhibits rice callus induction
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