Factors affecting the efficiency of serological methods for detection of blackgram mottle virus of mungbean
1985
Kittisak Kiratiya-angul
Mungbean mosaic virus was found in mungbean plants grown in some provinces of Thailand such as Nakornsawan, Kamphaengphet, Sukhothai. The virus was partially purified from infected mungbean plants and the yield of 25.1 mg/ml was obtained. The antiserum made by partially purified BGMV gave satisfactory reactions in the precipitin and agar gel double diffusion test resulting in the titer of 1:1024 and 1:512, respectively. By using agar gel double diffusion test, the suitable distance between antigens to react with the antiserum was 5-6 millimeters. Antiserum bands were clearer than r-globulin bands. Dropping of antiserum on the plant before antigens at least 6 hours showed the bands more sharply than dropping of antiserum and antigen at the same time. The precipitin test was found to be effective when using partially purified virus. The agar diffusion test showed the reaction clearly made possible and detection in 24 hours. For the IEM method, virus particles were detected from the sap of diseased leaves of mungbean samples. Antiserum diluted 1:1,000 to 1:10,000 detected good virus particles. By checking the symptoms on the mungbean leaves after inoculation, it was found that the use of the IEM method resulted in the detection of the virus particles in 3 days while the other two methods detected the reactions 4 days after inoculation. For the seed transmission of BGMV, 400 seeds from diseased plants were tested, 321 seeds were germinated and 3 plants (or 0.93 percent) were infected. Each of the 100 seeds was separated into its seedcoat, cotyledon and embryo. No virus particles of BGMV were detected by the IEM method
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