Toxicity of Fusarium isolates obtained from corn-producing area in Korea
1988
Lee, Y.W. | Kim, K.H. | Chung, H.S. (Seoul National Univ., Suwon (Korea R.). Coll. of Agriculture)
Ninety-one isolates of Fusarium species were isolated from corn and soil from 8 locations in Kangwon province, the major corn-producing area in Korea. The predominant Fusarium species isolated from corn seeds were F. graminearum and F. moniliforme whereas the major species from soil was F. equiseti. Percentage detection of Fusarium species from corn seeds ranged 11 to 94%. Each isolate was grown on 200g of autoclaved wheat grains for 2 weeks at 22-26 C, followed by 2 weeks at 10 C. The mass of fungus-invaded wheat was broken into pieces, allowed to air dry, and ground in a Waring blender. The toxicity was measured by feeding the cultures to twenty-one-day-old virgin female rats. The rats were observed frequently for 7 days, and the major symptoms and death were recorded. Thirty-three isolates killed all rats during the test period and caused intensive hemorrhages in stomach, intestines, and/or bladder. Intensive hemorrhages in internal organs were sometimes associated with uterus enlargement. In addition eleven isolates caused significant body weight loss, though they did not kill the rats during the test period. Toxicity values of total 91 isolates of Fusarium species were indicated by body weight loss and feed consumption. Correlation between body weight change and feed consumption seems to be useful for measuring the toxicity of Fusarium species
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