Experimental infection of infectious bovine rhinotracheitis virus to rabbits immunosuppressed with dexamethasone
1988
Min, W.G. | Jun, M.H. | Park, S.K. (Chungnam National Univ., Taejon (Korea R.). Coll. of Agriculture) | An, S.H. (Rural Development Administration, Anyang (Korea R.). Veterinary Research Institute) | Cha, Y.H. (Coongang Animal Disease Laboratory, Anyang (Korea R.))
To establish a laboratory animal model for study on development of diagnostic methods for infectious bovine rhinotracheitis virus(IBRV), experimental infection of the virus to rabbits immunosuppressed with dexamethasone(DX) were carried out. When lymphocyte activity was measured by lymphocyte transformation to phytohaemagglutinin parallel with total and differential leucocyte counting, both groups treated with 2.0mg DX once and 1.0mg DX daily showed marked immunosuppression between 5 to 72 hrs. after administration. The degree of suppression of lymphocyte activities was more remarkable in the latter group. IBRV PQ7 strain at 10** (7.5) TCID50/0.2ml was inoculated into conjunctival sacs of rabbits immunosuppressed with DX and non-treated. During 3 weeks observation, the immunosuppressed groups revealed mild conjunctivitis, viremia and virus recovery by 33.3 to 100%, whereas the DX nontreated group showed viremia and virus recovery with no clinical conjunctivitis in one of three rabbits(33.3%). In conclusion, it was indicated that experimental infection of IBRV PQ7 strain to rabbit was limited in prerequisite to immunologic modification by administration of immunosuppressive drugs
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