Benomyl as seed treatment to control storage fungi in corn [Zea mays; Philippines]
1987
Garcia, R.P.
Storage fungi (Aspergillus spp. and Penicillium spp.) isolates from corn seeds were inoculated in Czapek's and Czapek yeast autolysate agar infused with 0, 1, 2, 5 and 10 ppm Benomyl to evaluate its efficacy against these two fungal genera. The Aspergillus and Penicillium isolates' mycelial growth was increasingly inhibited in agar infused with 1, 2, and 5 ppm Benomyl. Neither isolate exhibited any growth in agar media infused with 10 ppm Benomyl. Conidial germination of the isolates was generally suppressed by 5 and 10 ppm Benomyl. The degree of inhibition of growth, sporulation and conidial germination progressed with increasing concentrations of the fungicide. Corn seeds with 12.7 and 16.5% moisture were inoculated with agar discs of storage fungi and treated with different rates of Benomyl. To test the protective and eradicative effects of the fungicide, some grains were treated with Benomyl two days before and after inoculation, respectively, and stored for 3, 6 and 9 weeks. Corn seeds with 12.7 and 16.5% moisture treated with 5 g formulation/kg seeds had significantly lower mean storage fungi infection. Also, aflatoxin contamination and benomyl residues were not detected. A. flavus mean kernel infection was significantly higher than P. cyclopium in both protective and eradicative treatments as well as under consideration of moisture contents, all concentrations of benomyl and storage periods.
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