Rapid and sensitive method to estimate salinity tolerance of Azolla pinnata
1987
Rajarathinam, K. | Padhya, M.A. (M.S. Univ of Baroda, Baroda (India). Botany Dept.)
A rapid, nondestructive but sensitive method for assessing the salinity tolerance of different strains of Azolla pinnata have been developed. Pigments (chlorophyll and phycocyanin) are measured by their fluorescence in the heterocysts and the vegetative cells of Anabaena azolla isolated from sodium chloride-treated azolla-using Leitz fluorescence microscope. Chlorophyll and phycocyanin were reduced in both types of cells. The acetylene reduction activity of the azolla-anabaena complex also showed reduction, which correlated with that in the pigments of the endophyte. Measuring the chlorophyll and phycocyanin content of anabaena isolated from salt-treated azolla can be used to estimate the salinity tolerance of a particular strain of azolla.
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