Simple and convenient method to preserve seed of rice germplasm
1987
Chang-Xiang Mao (Hunan Hybrid Rice Research Center, Changsha, Hunan (China))
More than 500 samples of rice seed harvested in autumn 1976 were sealed with indicating silica gel in glass ampules. The sealed ampules were sorted in boxes placed in an ordinary room where the average monthly temperatures are about 5 degrees Celsius, 6 degrees Celsius, 11 degrees, 17 degrees, 22 degrees, 26 degrees, 29 degrees, 24 degrees, 18 degrees, 12 degrees, and 10 degrees Celsius Jan-Dec. After 10 yr, the seeds still have desirable germination abilities. Average moisture content before sealing was about 12% it is now 7-8%. Average germination rate is about 90%. Seedlings derived from the germinated seeds grow well in the field. The characteristics of this preservation method are: (1) well-sealed conditions. The ampule was sealed by melting the glass tip, the relative humidity was very low and, with adequate silica gel, continuously becomes lower and lower; (2) Ease in operating. It is very easy to seal the ampule vials using a bunsen burner and a pair of tweezers; (3) Ease in keeping. The ampule is made of glass, which cannot be affected by moisture, insects, and rats. It can be stored in normal conditions; (4) Low cost. The ampules are very cheap. One 2-ml ampule can store 20-30 rice seeds, enough to use for breeding parents. A 20-ml ampule bottle can hold 500 rice seeds; (5) Use for other crops. Glass ampules can be used for other small-seed crops, such as wheat, vegetables, and rape seeds.
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