Studies on embryo transfer in the pig, 1; Effect of days and repeat collection on the embryo production
1990
Kim, H.S. (Rural Development Administration, Suwon (Korea R.). Livestock Experiment Station) | Toshiyuki Kojima | Tadashi Soma (National Inst. of Animal Industry, (Japan))
This study was carried out to investigate the effect of collection days of embryos, surgery times by repeated recovery, and number of follicles on the number of embryos recovered, developmental stage and number of transferable embryos in order to provide the basic data for the surgical collection of pig embryos. The superovulation was induced by PMSG 1,000IU and HCG 500IU on day 15 to 16 of estrus cycle. The average number of embryos collected on day 4, 5 and 6 following HCG injection were 13.1, 17.1 and 12.7, respectively, and recovery rate was 97.8, 96.6 and 81.9 %. Embryos collected on day were best in the recovery rate and number of embryos collected. In the developmental stage by recovery days, the percentages of 2-to 16-cell on day 4 morula to blastocyst on day 5 blastocyst to hatched blastocyst on day 6 were 28.2 %, 38.0 % and 61 % respectively. The abnormal rates of embryos collected on day 4, 5 and 6 were 43.6 %, 13.9 %, and 23.6 % respectively. The average number of embryos collected by the no. of operations for the repeated collection was 19.2, 13.0 and 13.7 in 1st and 2nd surgery, 3rd and 4th and over 5th surgery, respectively. The number of embryos collected was most in 1st to 2nd surgery but the recovery rate of it was slightly lower. The percentages of transferable embryos by repeated operation were 42.2 %, 76.9 % and 75.9 % in 1st and 2nd, 3rd and 4th and over 5th operation respectively, but there were no significant differences in the number of transferable embryos among the operation times. The number of embryos collected from ovaries without unruptured follicle was 16.1 and the recovery rate was 94.7 % and the number and the percentages of normal embryos were 12.3 and 74.6 % respectively which were higher than those from ovaries with the unruptured follicles
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