Studies on the development of photoreceptor in the nonchromatophore organisms 4., Effects of organic compound and metal ion influx of light-induced mitochondrial ATP synthase in Lentinus edodes (Berk.) sing
1989
Min, T.J. | Lee, W.G. (Tongguk Univ., Seoul (Korea R.). Coll. of Sciences) | Kim, J.W. (Yuhan Technical Coll., Seoul (Korea R.). Dept of Food Nutrition) | Min, T.I. (Korea Advanced Institute of Science and Technology, Seoul (Korea R.))
Effects of organic compounds, photosensitizers and influx of metal ions on the light-induced mitochondrial ATP synthase in Lentinus edodes purified by stepped sucrose density gradient centrifugation were studied. In our previous work, the activation wavelength and the illumination time of mitochondrial ATP synthase were 470 nm and 15 sec, respectively. This enzyme was activated 85 % by 1 mmole 2,6-dichlorophenol indophenol and inhibited by 1 mmole 2,4-dinitrophenol, 10 micro mole 2-heptyl-4-hydroxyquinoline-N-oxide and 100 micro g oligomycin per ml of ethanol. Particularly, the enzyme was activated 414 % by 10 mmole phenazine methosulfate as photosensitizer at 470 nm light. In the influx effects of Fe** (3)+ and Fe** (2)+ ion, the activity of the above enzyme increased under the optimal light condition compared with nonillumination state
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