Effect of GA3 and ABA on endogenous starch content during shoot differentiation in Cymbidium spp. protocorm
1988
Han, T.J. | Kang, Y.H. (Hallym Univ., Choonchun (Korea R.). Dept. of Biology) | Kim, E.S. (Yonsei Univ., Seoul (Korea R.). Dept. of Biology)
Changes in starch content and activities of ADPG-and UDPG-starch synthase and alpha- and beta-amylase were studied in order to investigate effects of gibberellic acid and abscisic acid on endogenous starch content during shoot differentiation and protocorm propagation in Cymbidium spp. (jungfrau) protocorm. Shoot differentiation was promoted during the degradation of endogenous starch and protocorm propagation was promoted during starch accumulation in protocorm. The activities of ADPG- and UDPG-starch synthase and alpha- and beta-amylase seemed to be related with starch content. Shoot differentiation and protocorm propagation were slightly inhibited in protocorm explants treated with 100micron M gibberellic acid. The explants treated with 10micron M abscisic acid lost the capacity for shoot differentiation and protocorm propagation, and that could not be overcome by 100micron M gibberellic acid added to culture medium. Starch content fluctuated as the control even after 10micron M abscisic acid. Nonetheless, the treatment completely inhibited shoot differentiation and protocorm propagation
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