Levels of plasma progesterone, estradiol-17-beta and several serum chemical components in recipients at the time of nonsurgical transfer of frozen/thawed bovine embryos
1989
Lee, B.C. | Jo, C.H. | Hwang, W.S. (Seoul National Univ., Suwon (Korea R.). Coll. of Veterinary Medicine)
A total of 13 synchronized dairy cattle (Holstein) were used to determine pregnancy rates in relation to plasma progesterone, estradiol-17-beta levels and serum chemical values on the day of last PGF 2 alpha injection and day of frozen/thawed bovine embryo transfer. The pregnancy rate of recipients with 1.0-4.0ng/ml of progesterone levels at the day of last PGF 2 alpha injection was higher than that of recipients with below 1.0ng/ml or above 4.0ng/ml of progesterone levels. On the day of transfer, optimal progesterone levels were between 1.0ng/ml and 4.0ng/ml coinciding with a pregnancy rate of 88.9 %. Pregnancy rate decreased when progesterone levels were below 1.0ng/ml (33.3 %) or above 4.0ng/ml (0 %). Corpus luteum grade did not affect pregnancy rate and this result revealed that manual palpation of corpus luteum was not valid criterion of corpus luteum function. Progesterone levels as well as pregnancy rate did not significantly differ whether the corpus luteum was on the right (1.62 +- 1.33ng/ml; 63.5 %) or left ovary (1.99 +- 0.61ng/ml; 85.0 %). Estradiol-17-beta levels were not significantly different between pregnant and nonpregnant recipients, but estradiol-17-beta levels (82.2 +- 13.5 VS. 72.3 +- 10.1pg/ml) were higher at below 1.0 ng/ml of progesterone. Total cholesterol levels on the day last PGF 2 alpha injection and day of transfer did not affect pregnancy rate. Calcium and inorganic phoshorus levels belonged to normal range in most of the recipients. These range did not affect pregnancy rate. In reviewing above results, plasma progesterone levels (1.0-4.0ng/ml) at the time of transfer are diagnostic value for screening recipients prior to transfer of frozen/thawed bovine embryos
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