Biodegradation of cellulose by wood rotting fungi: carboxymethyl cellulase from Lenzites sepiaria, a brown rotter
1991
Bhattacharjee, B.K. | Roy, A. | Majumder, A.L.
The aim of the present work is to study the physiology and biochemistry of cellulose degradation by wood rotting Basidiomycetes fungi. From a survey of 20 species of fungi for their potentiality of cellulase production 2 species namely, Lenzites sepiaria, a brown rotter and Polyporus xeranticus, a white rotter, were screened out as best produce and subjected for further study. As all the wood rotting microorganisms produce abundant endoglucanase, initial study was restricted to this enzyme only. Both these strains produced appreciable amount of endoglucanase and xylanase when the growth medium (Czapek- Dox) contained any one of Sigma Cell Type 20, Cotton, Filter paper dust or carboxymethyl cellulose (CMC), each at the concentration of 1%. Purification of the endoglucanase from L. sepiaria was accomplished by a combination of ammonium sulphate streptomycin sulfate precipitation, followed by Gel filtration chromatography and was found homogeneous in 10% PAGE. The purified enzyme was only active towards CMC. Nitrogen and glucose at different concentration had no effect on the production of endoglucanase during growth. The purified CMCase from L. sepiaria has been characterized.
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