Characterization of recombinant derivatives of pJY711 of multicopy Streptomyces plasmid
1990
Yum, D.Y. | Kong, I.S. | Yu, J.H. (Yonsei Univ., Seoul (Korea Republic). Coll. of Specializing in Engineering)
The restriction cleavage map of multi-copy recombinant plasmid, pJY712 (8.1kb), carrying the thiostrepton resistance gene (tsr) was determined. pJY712 had a broad host range in Streptomyces and contained single Bg/II site for cloning purpose. The plasmid showed the phenomenon of lethal zygosis (Ltz +). Transformation frequency of pJY712 was 5.0 x 10** (4) transformants per ug plasmid DNA (TFU) in S. lividans. Plasmid pJY713 was constructed by inserting the tyrosinase gene (mel) into teh C/I site of pJY712. Recombinant plasmid pJY714 carrying the mel gene was constructed by in vitro deletion of a segment (1.9 kb Bg/II-Bc/I fragment) from pJY713
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