Patterns of protein synthesis during the second cleavage of mouse two-cell embryos: Effects of colcemid and alpha-amanitin
1989
Kang, H.M. | Kim, K.J. | Cho, W.K. (Seoul National Univ., Seoul (Korea Republic). Coll. of Natural Sciences) | Lee, K.K. (Korea Advanced Inst. of Science and Technology, Seoul (Korea Republic). Genetic Engeering Center)
In this study, we attempted to determine the precise patterns of protein synthesis during the second cleavage in mouse. F1 hybrid 2-cell embryos showing a highly synchronized cell cycle and outbreed ICR strain 2-cell embryos were used. The patterns of protein synthesis during the second cleavage showed the sequential changes in the F1 hybrid and ICR strain 2-cell embryos. Moreover, we examined the effects of mitotic and transcriptional inhibitors such as colcemid and alpha-amanitin on the protein synthesis in the late 2-cell embryos of ICR strain. Treatment of colcemid (0.1 micro g/ml) blocked the second cleavage, but did not affect on the change of protein synthesis. However, treatment of alpha-amanitin induced the synthesis of two sets of polypeptides without affecting on synthesis of other proteins and cleavage. It thus seems that the appearance of alpha-amanitin-sensitive proteins may be not involved in the second cleavage. Therefore, these results indicate that the second cell cycle in mouse embryos appears to be regulated at post transcriptional level, presumably independent on the expression of embryonic genome
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