Biological control of bacterial wilt on white potato with avirulent bacteriocin producing strains of Pseudomonas solanacearum [Philippines]
1989
Juan, Z.D.
The experiment was conducted to: (1) identify Pseudomonas solanacearum biovars present in Benguet and Ilocos Norte [Philippines]; (2) bacteriocin producing strains of P. solanacearum among the isolates collected; and (3) assess the protection of potatoes from bacterial wilt with the selected strain from 20 strains using seed potatoes and stem cuttings. Biovars 2 and 3 were isolated from collections in Benguet, and only biovar 3 from those collected in Ilocos Norte. The selected bacteriocin-producing strain was isolated from white potato against an isolate from tomato 1 both of biovar 3. Seed potatoes and stem cuttings (IPB Selection 1) treated with 5 x 10 to the eighth power cells/ml ABPS [Avirulent Bacteriocin-Producing Strains] effected the least number of wilted plants and least degree of wilting. This concentration delayed the occurrence of wilting for two weeks as compared to lower concentrations of ABPS. Plants grown from stem cuttings' wilted two weeks earlier than those from seed potatoes. Stem cuttings treated with 4 x 10 to the eighth power cells/ml ABPS and planted 12 days after ABPS drenching had the least number of wilted plants and least degree of wilting as compared to plants planted 0, 3 and 6 days after ABPS drenching. Results are indicative that the use of ABPS delayed the occurrence of bacterial wilt. However, the highest concentration used is still low to effect adequate cross protection from the virulent strain of the pathogen.
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