Using anther culture to develop parental lines for hybrid rice breeding
1989
Xu, D. | Wang, M. | Song, R. (Agricultural Research Inst. of Shuangfeng County, Hunan (China))
Anthers at the uninucleate pollen stage of V20 A (Yebai A line), V20 B (B line), and IR26 (R line) were cultured in vitro. Pollen grains of the A line gave rise to pollen plants. However, green plant induction was low compared with that of the R and B lines. Pollen plant induction of the F1 hybrids of the crosses V20 A/IR26 and V20 B/IR26 was higher than those of the R and B lines. Pollen lines derived from the A line segregated, perhaps because of heterozygous sterility in V20 A. This heterozygosity postponed pollen abortion in V20 A longer than in the original Yebai A line. When its homozygosity was recovered by anther culture, some segregants resumed the original sterility characteristics. Pollen plants derived from culturing anthers of V20 B and IR26 mostly resembled the B and R lines. Some mutants occurred, which might be due to somaclonal variation occurring in anther culture or to residual heterozygosity in the experimental materials. Through anther culture, homozygosity can be achieved quickly and sterility can be identified in the first generation.
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