Microflora associated with outdoor cultivation of Volvariella volvacea (Bull. Ex. Fr.) Sing
1988
Petcharat, V.
Fifty-five species (433 isolates, 37 genera) of fungi, three hundred and seventy-six isolates of unidentified bacteria and ninety-four isolates of filamentous actinomycetes were collected. The percentage frequency of occurrence of each identified fungus was calculated at various stages of bedding. Verticillium sp. was most dominant at the beginning of bedding while Aspergillus fumigatus was most common after harvesting. Relative cellulolytic activity of each of the fungus was measured and thirteen species of mesophilic and five species of thermophilic fungi showed strong cellulolytic activity. Volvariella volvacea mycelium were grown in dual cultures with each isolated organism and in media supplemented with culture filtrate of selected organism to observe antagonistic and/or enhancing effects. Thermoactinomycete isolate TA-1 promoted growth of mushroom mycelium probably by producing some metabolites secreted into the medium. Changes in the population of each group of microorganism, pH, moisture, and temperature within the straw mushroom were investigated throughout bedding time. The pH increased very fast during the first and second days of bedding, after which there was a slight change, while the moisture content decreased towards the end of the bedding time. The general pattern found in this study was an increase in temperature during bedding time, stimulating growth of thermophiles.
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