Methods for culturing stored grain insects
1989
Wright, V.F. | Mills, R.B. | Willcutts, B.J. (Kansas State University, Manhattan, KA (USA). Department of Entomology)
Stored-grain insects can be cultured with relative ease. However, for research purposes, care must be taken to schedule culture time and avoid overpopulation of cultures, disease, parasites, and other stresses. Particular attention should be given to sanitation in the culture room and avoidance of cross-contamination, especially from grain that has not been disinfested. For reproducibility of results, insects must be unstressed, of similar genetic background, and free of disease. Conditions in the culture room should be standardized and consistent for temperature and relative humidity. Understanding details and differences in biology and behavior of stored-product insect pests will allow testing for resistant varieties to be carried out with reasonable results. Detailed procedures are given for general stored-product culturing. Specific methodologies are described for grain weevils (Sitophilus sp.), grain borers (Rhyzopertha dominica (Fabricius) and Prostephanus truncatus (Horn)), grain moths (Plodia interpunctella (Hubner) and Sitotroga cerealella (Olivier)), and grain beetles (Tribolium castaneum (Herbst) and Trogoderma granarium Everest).
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