Influence of host virus content on the acquisition and transmission of barley yellow dwarf virus
1990
Nazare Pereira, A.M. | Lister, R.M. | Barbara, D.J. | Shaner, G.E. (Purdue University, West Lafayette, IN (USA))
Oats (Avena sativa L.), wheat (Triticum aestivum L.), and barley (Hordeum vulgare L.) showed wide variations in barley yellow dwarf virus (BYDV) content, as assessed by enzyme-linked immunosorbent assay (ELISA), among different leaves on the same plant, and also between leaves in the same position (i.e., of the same age) on different plants. Selected entire plants and individual leaves were compared as virus sources for the acquisition and transmission of BYDV isolates-Purdue (P)-PAV, MAV, and RPV-by specific (efficient) or nonspecific (inefficient) vectors. Overall, there was no convincing evidence of differences in virus acquisition efficiency due to differences in virus content in leaves or plants. By contrast, in experiments where aphids fed on purified virus preparations through membranes, correlation between virus concentration and virus acquisition (as assessed by ELISA of aphid extracts and by transmission tests) was evident for both efficient and inefficient vectors. Also, aphids apparently acquired virus more individual leaves containing the same overall virus concentration. The combined results suggest that total BYDV content is not the primary factor affecting the acquisition of BYDV from plants by vectors, but that acquisition is probably strongly influenced by other factors, especially uneven distribution of virus within the leaf.
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