The use of cloned hybridization probes to detect barley yellow dwarf virus infection in tetraploid wheat germplasm
1990
Pace, C. de | Delre, V. | Porceddu, E. | Casa, R. (Universita della Tuscia, Viterbo (Italy))
A PAV-like isolate of barley yellow dwarf virus (BYDV) was used in controlled inoculation experiments in the greenhouse to determine the intensity of the autoradiographic signal from positive Northern dot-blot hybridization of leaf sample extract of infected and uninfected plants of tetraploid wheat (Triticum turgidum L.). Radioactive probes gave more consistent results than biotin probes. The proportion of entries with reduced virus content in collections from Ethiopia, Iran, Iraq, and Turkey were 24.3, 28.6, 8.7 and 10.5 %, respectively. No other differential symptoms were expressed on the infested plants in the greenhouse. Therefore, the further evaluation of the tetraploid wheat entries for tolerance should involve (a) field testing and (b) the use of additional criteria to evaluate tolerance to BYDV.
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