Purification and application of a virus-inhibiting substance from Chenopodium amaranticolor
1983
Klas, F.E. | Soerdjlal-Sital, R. (Agricultural Experiment Station, Paramaribo (Suriname). Dept. of Virology)
A virus inhibiting substance was purified from leaves of Chenopodium amaranticolor, by subsequent 50 % saturation with ammonium sulphate, distillation over an oil bath, and column chromatography. The partition coefficient (Kav) of the substance was estimated to be 0.25. Consequently a molecular weight of 29,000 daltons was found. In both, 'preventive' and 'curative' experiments, using half leaves of Nicotiana glutinosa, the relative inhibition, with respect to eggplant mosaic virus, was very high. In experiments with N. glutinosa and Brassica juncea (Coss.) Kaisoi plants, the purified inhibiting substance proved to be phytotoxic at higher concentrations. On the other hand, soil application of the substance was not a success, since it was not taken up by the plant. Taking into consideration that the in vitro experiments gave better results, the inhibiting substance will further be applied in tissue culture procedures to obtain virus-free explant.
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