Androgenesis of certain maize genotypes
1989
Nikolic, Dj. (Maize Research Institute "Zemun Polje", Beograd (Yugoslavia)) | Knezevic, T. | Bogicevic, M. | Surlan, G. | Neskovic, M.
The anthers of eight maize genotypes were planted on Yu-pei (YP) medium, during the uni-nucleate microspore stage of development. The anthers had previously been exposed to a cold treatment of 14 days at 8'C. After five to eight weeks the first androgenic calli were noticed with the 78-01 genotype. The remaining genotypes showed no response to the same treatment. Upon transferring embryogenic calli to YPNAS-10 regeneration medium, numerous plants were regenerated. The regeneration medium containing macro-and micro-elements also contained 25 mgl-1 succinic acid, 0.25 mgl-1 aba and l mgl-1 kinetin. Most of the regenerated plants showed normal morphological characteristics during the early stages of growth.
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