Histochemistry of acid phosphatase in the small intestine mucosa during experimental coccidiosis of sucking piglets
1991
Kudweis, M. (Nemocnice, Cesky Krumlov (CSFR). Vyzkumna a Diagnosticka Laborator) | Lojda, Z. | Julis, I.
The activity of acid phosphatase was evaluated densitometrically in the mucosa of duodenum, jejunum and ileum of 22 conventional piglets which were experimentally infected by oocysts of the coccidiae Isospora suis on day one after parturition. The activity of the studied hydrolase was investigated in the infected piglets during days two to ten after infection in the intestinal mucosa and in goblet cells. The density of the reaction product of acid phosphatase was simultaneously determined in the same mucosal cells of different sections of the small intestine in five control conventional piglets at the age of 2-14 days. Important changes were revealed, in comparison with the control data, in the development of the activity of acid phosphatase in the intestinal mucosa cells in the experimentally infected piglets. In the period of investigation there were two stages of the development of the density of the enzyme reaction product. The first stage can be characterized by an increase, the other by a decrease in the level of acid phosphatase activity. Enterocytes are influenced in both stages, but the decrease in the density of the reaction product of acid phosphatase was observed only in absorption cells, and not in goblet cells.
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