The effects of some antibiotics on microorganisms contaminating boar ejaculates
1991
Mazurova, J. (Statni Veterinarni Ustav, Pardubice (CSFR)) | Vinter, P.
The occurrence of microorganisms, including their total counts in boar native ejaculates, was investigated in two stages; the objective of this investigation also was to determine contamination after the sperms were treated with diluents containing the antibiotics ampicillin, gentamycin, apramycin, cefoxitin, or antibiotic combinations penicillin + streptomycin, ampicillin + cefoxitin, gentamycin + cefoxitin and ampicillin + gentamycin. The representation of bacterial species and total counts of microbes in sperm were determined in 24, 48 and 72 h after dilution. Proteus sp. (63.3 per cent) and Pseudomonas aeruginosa (51.5 per cent of the total number of examined samples) were the most frequent species. The number of contaminated diluted ejaculates ranged from 12.5 to 95.8 per cent in 24 h after dilution, from 12.5 to 98.5 per cent in 48 h and from 16.8 to 95.8 per cent of the total number of examined ejaculates in 72 h. The occurrence of microorganisms correlated mostly with the efficiency spectrum of the antibiotics or their combinations. The highest average counts in 1 ml of diluted sperm were found in ejaculates containing cefoxitin and apramycin. Gentamycin was the most effective antibiotic used as a sole component (average counts of microorganisms CPM in 1 ml were 416 in 24 h, 955 in 48 h and 2260 in 72 h after dilution); ampicillin and gentamycin were the most efficient combination (14-20-21). This combination exerted very good effects also on Proteus sp. and Pseudomonas aeruginosa.
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