The histochemistry of the nucleoside phosphatases and phosphoglucomutase of the small intestine of piglets suffering from coccidiosis
1991
Kudweis, M. (Nemocnice, Cesky Krumlov (CSFR). Vyzkumna a Diagnosticka Histochemicka Laborator) | Lojda, Z. | Julis, I.
The first day after birth, 22 conventional piglets were experimentally infected with the oocysts of the coccidia of I. suis (infection dose 200 000 oocysts). The activity of 5-nucleotidase (5-ribonucleotid phosphohydrolase, EC.3.1.3.5.) and phosphoglucomutase (alpha-D-glucoso-1-phosphat phosphotransferase, EC.5.4.2.2.) was densitometrically assessed in the mucosa of the small intestines of these piglets. Enzyme activities were studied in the infected piglets during the 2nd to 10th day after infection. The same histochemical examination was simultaneously performed in the intestinal mucosa of five control conventional piglets at an age of 2-14 days. 5-nucleotidase and phosphoglucomutase were found to have a high density in the mucosa of the small intestine of the control piglets: the high-density locations of these enzymes include, first of all, the supranuclear area of the absorption cells, the microvillous zone of enterocytes and the smooth muscle elements of lamina muscularis mucosae. The experimentally infected piglets showed a marked decline of the density of both enzymes during the infection. The deficit affected, for a transient period, the microvillous zone and the supranuclear region of enterocytes; the musculature of the mucous layer was affected permanently. The inactivity was more protracted in the case phosphoglutamase (especially 5 to 9 days after infection). The density of 5-nucleotidase showed a partial return to the normal already the 7th day after infection, with an interruption of resumption of activity on the 10th day. Resumption of enzyme activity in the lamina muscularis mucosae was not recorded during the infection. In the three locations under study, the density of none of the enzymes did reach parameters comparable with the controls at the end of the trial (10 days after infection).
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