Clonal multiplication of ramie by tissue culture
1991
Del Rosario, A.G. | Corcolon, B.M. (Philippines Univ., Los Banos, College, Laguna (Philippines). Dept. of Horticulture)
A study on the culture of ramie shoot tip and nodal explants was conducted to develop a tissue culture technique for its mass propagation. The most effective surface sterilization procedure tested was dipping the explants on ethanol, Benomyl and Calcium Hypochlorite solution. Callus formation and occasional shoot development was induced in shoot tip and nodal sections in medium with indoleacetic alone or in combination with benzylademine, kinetic or 2-isopentenylademine. Shoot induction within two weeks was observed when the explants were cultured on MS medium supplemented with naphthalene acetic acid and kinetin. Single node microcuttings from regenerated plantlets are still capable of further shoot multiplication and regeneration. A 100% survival rate was observed among the rooted plantlets transferred to 1:1 soil/sand potting mixture under greenhouse conditions.
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