Large scale production of in vitro potato tubers: an example
1991
Li, Bao Qin | Zheng, Miaochang | Luo, Xi Jin | Wang, Jun | Song, Bofu (South China Agriculture Univ., Guang Zhou, Guangdong (China))
Guangdong, the warmest province in China is able to export large quantities of potatoes to Hongkong. With seed tuber imports from the Netherlands no longer permitted, Zongshan City developed a factory to produce virus-free in vitro tubers of the most popular new variety Golden Crown. A simple methodology was developed whereby 5 to 6 two-node cuttings are inoculated into 150 ml bottles containing 5 to 7 ml of MS media for multiplication of shoots. Once the desired number of shoots are obtained, these are switched over to a tuber inducing medium and placed in continuous darkness for 30 days. This produced an average of 10 microtubers/bottle. Five million microtubers were produced during 1988 and 1989. These have been sold to a Hebei Government seed farm, Zhongshan City and field multiplied to produce seed tubers for commercial potato production. The cost of production is such that with the present staff and facilities the factory must produce 10 million microtubers per year to make a slight profit. Details of the whole process are discussed.
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