Studies on strains of tobacco mosaic virus by symptom, host range, serology and cross protection test
1987
Suvalux Chaichuchot
Some properties of various isolates of tobacco mosaic virus were examined. Virus isolated from tobacco (isolate T), tomato (isolate To)and pepper (isolate P) showed different symptoms on test plant. T isolate produced light and dark green mosaic and slight crinkle. To isolate produced necrotic local lesion on inoculated leaves. P isolate produced mild light and dark green mosaic and malformation. Virus isolated from tobacco, tomato and pepper showed different symptoms on Nicotiana tabacum Linn. T isolate produced mosaic and slightly crinkle leaves. To isolate produced necrotic local lesion on inoculated leaves. P isolate produced mild mosaic and malformation. T-4 isolate produced severe green and yellow mosaic leaves. T-1, T-2 and T-3 isolates produced mosaic. Types and symptom of host range of various isolates were similar to each other. They caused either systemic reaction or local reaction on plants from Amaranthaceae, Chenopodiaceae and Solanaceae. Serological relationships among these isolates were determined against antiserum of T isolate by using agas-gel double diffusion test and immunoelectron microscopic method (IEM). To, p, t-1 and T-3 isolates were identically related to T isolate where T-2 and T-4 isolate were distantly related to T isolate on double diffusion test. IEM were also able to trap virus particles of these testing isolates, with no difference on the number of trapped particles, when antiserum against T isolate was employed. In cross protection test prior infection with tobacco isolate was found to prevent subsequent infection by tomato isolate or pepper isolate. Any plant that is infected with pepper isolate was found to prevent subsequent infection by tomato isolate.
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