Photosystem 2 remains capable of oxygen evolution after losing one-fourth of its initial manganese content
1991
Mavankal, G. | McCain, D.C. (Southern Mississippi Univ., Hattiesburg, MS (USA))
Photosystem 2 (PS2) membranes were treated with calcium chloride to remove extrinsic polypeptides. After resuspension in a chloride-free buffer, manganese was extracted by leaching or with EDTA. Measuruments made using three different methods (electron paramagnetic resonance, kinetics, and direct Mn analysis) agreed that one-fourth of the initial Mn content was lost and that the membrane preparation remained homogenous. Upon readdition of extrinsic polypeptides, the Mn-depleted membranes were capable of oxygen evolution. Thus not all the Mn in PS2 is necessary for the oxygen evolution function.
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