In vitro cultivation of plant parasitic nematodes
1992
Hadbavnikova, J. | Sabova, M. (Slovenska Akademia Vied, Kosice (CSFR). Helmintologicky Ustav)
Aphelenchoides fragariae was cultivated in vitro on strawberries as well as on callus cultures. Much higher reproduction of these nematodes was reached on alfalfa calluses. After 3 weeks of inoculation about 100 nematodes to callus their number increased to 25000. At present we carry out in vitro cultivation of the following plant parasitic nematodes species coming from various countries: Aphelenchoides fragariae, and A. ritzemabosi, Ditylenchus dipsaci of alfalfa and red clover nematoda and Pratylenchus penetrans.
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